|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 226-271 of human PITPNB|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 32 kDa.
Purity is >95% by SDS-PAGE.
The lipid binding proteins known as phosphatidylinositol transfer proteins (PITP) facilitate the formation of phosphatidylinositol derived second messenger molecules, which are related to the phospholipase C and phosphoinositide 3-kinase pathways. PITP are ubiquitously expressed proteins that transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes enriched in PI or PC to membranes that are deficient in PI or PC. PITP mobilizes PI from the endoplasmic recticulum and regulates the release of PI from stored vesicles in the Golgi network. In mammalian cells, three smaller forms of soluble PITP are present, designated PITP alpha, PITP beta and retinal degeneration B (rdgB) beta. PITP beta is a 271 amino acid protein that is widely expressed in various tissues. Though required for Golgi targeting, constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity. There are two isoforms of PITP beta that are produced as a result of alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.