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Invitrogen
PIWIL1 Polyclonal Antibody
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FIGURE: 1 / 1
PIWIL1 Antibody (PA5-17030) in WB
Western Blot was performed using Anti-PIWIL1 Polyclonal Antibody (Product # PA5-17030) and a 100 kDa band corresponding to Piwi-like protein 1 was observed across the tissues tested except Mouse Liver and Mouse Heart along with uncharcterized bands ~50 kDa. Whole cell extracts (30 µg lysate) of Mouse Testis (Lane 1), Mouse Liver (Lane 2), Mouse Ovary (Lane 3), Mouse Heart (Lane 4) and Rat Testis (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a PVDF ... View More
Product Details
PA5-17030
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Asp478 of mouse Miwi
Conjugate
Form
Liquid
Concentration
62 µg/mL
Amount
6.2 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34- hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CT80; HILI; member of the PIWI family; mili; Miwi like; Miwi likw; piwi homolog; piwi like homolog 1; piwi-like 1; piwi-like homolog 1; Piwi-like protein 1; piwi-like protein 2; PIWIL1L; piwil2-like protein; unnamed protein product
Gene Aliases: CT80.1; HIWI; MIWI; PIWI; PIWIL1
UniProt ID: (Human) Q96J94, (Mouse) Q9JMB7
Entrez Gene ID: (Human) 9271, (Rat) 363912, (Mouse) 57749
regulation of translation
spermatogenesis
spermatid development
cell differentiation
gene silencing by RNA
piRNA metabolic process
sperm chromatin condensation
meiotic cell cycle
primary piRNA processing
piRNA-mediated gene silencing by mRNA destabilization
transposable element silencing by piRNA-mediated heterochromatin formation
pachytene
multicellular organism development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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