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Western blot analysis of Mouse Liver Tissue Lysate using PA5-19457, PIWIL1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19457 staining of Rat Testis Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from within residues 800 - 900 of Human PIWIL1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19457 targets PIWIL1 in WB applications and shows reactivity with mouse and Rat samples.
The PA5-19457 immunogen is synthetic peptide derived from within residues 800 - 900 of Human PIWIL1.
PA5-19457 detects PIWIL1 which has a predicted molecular weight of approximately 99 kDa.
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIWI, MIWI, PIWI, piwi homolog; piwi homolog; piwi like homolog 1; piwi-like 1; piwi-like homolog 1; piwi-like protein 1; piwi-like RNA-mediated gene silencing 1
CT80.1; HIWI; MIWI; PIWI; PIWIL1