Western blot analysis of Mouse Liver Tissue Lysate using PA5-19457, PIWIL1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19457 staining of Rat Testis Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from within residues 800 - 900 of Human PIWIL1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19457 targets PIWIL1 in WB applications and shows reactivity with mouse and Rat samples.
The PA5-19457 immunogen is synthetic peptide derived from within residues 800 - 900 of Human PIWIL1.
PA5-19457 detects PIWIL1 which has a predicted molecular weight of approximately 99 kDa.
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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