Invitrogen

PIWIL4 Polyclonal Antibody

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PIWIL4 Antibody in Western Blot (WB) — Western blot analysis of PIWIL4 using PIWIL4 Polyclonal Antibody (Product # PA5-116559) at a 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-116559

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIWIL4 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.78 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive Samples: SH-SY5Y, Mouse testis, Mouse spleen, Rat testis, Rat spleen

Target Information

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mAgo5; piwi-like 4; piwi-like homolog 4; Piwi-like protein 4; piwi-like RNA-mediated gene silencing 4; testis tissue sperm-binding protein Li 85P

Gene Aliases: 9230101H05Rik; HIWI2; MIWI2; PIWI; PIWIL4

UniProt ID: (Human) Q7Z3Z4

Entrez Gene ID: (Human) 143689, (Mouse) 330890, (Rat) 689972

Molecular Function: translation initiation factor