Western Blot analysis of PKA alpha in HeLa lysate (35 µg protein in RIPA buffer) using PKA alpha Polyclonal Antibody (Product # PA5-143276) at 2 µg/mL incubated for 1 hour. Detected by chemiluminescence.

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Product Details

PA5-143276

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-KHKETGNHYAMK.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2933915

Product Specific Information

Additional Information: This antibody is expected to recognize all three reported isoforms (NP_002721.1; NP_997401.1; NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C(s); C-alpha-2; C-alpha-S; C-alpha-s; alternatively spliced isoform; cAMP-dependent protein kinase catalytic subunit alpha; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; unnamed protein product

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Gene Aliases: CAFD1; PKACA; PPNAD4; PRKACA

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UniProt ID: (Human) P17612

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Entrez Gene ID: (Human) 5566

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Function(s)

nucleotide binding magnesium ion binding protein kinase activity protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding protein domain specific binding manganese ion binding ubiquitin protein ligase binding protein kinase A regulatory subunit binding channel activator activity protein serine kinase activity

Process(es)

mesoderm formation neural tube closure regulation of heart rate renal water homeostasis mRNA processing protein phosphorylation protein export from nucleus adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of macroautophagy peptidyl-serine phosphorylation cytokine-mediated signaling pathway cellular potassium ion homeostasis cellular response to nutrient levels positive regulation of insulin secretion negative regulation of interleukin-2 production high-density lipoprotein particle assembly cellular response to heat mitochondrial protein catabolic process interleukin-2-mediated signaling pathway TORC1 signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process regulation of osteoblast differentiation positive regulation of gluconeogenesis negative regulation of smoothened signaling pathway positive regulation of protein export from nucleus sperm capacitation positive regulation of phagocytosis modulation of synaptic transmission positive regulation of calcium-mediated signaling regulation of cell cycle regulation of cardiac muscle contraction regulation of proteasomal protein catabolic process cellular response to cold regulation of protein processing cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction vascular endothelial cell response to laminar fluid shear stress postsynaptic modulation of chemical synaptic transmission cAMP/PKA signal transduction regulation of cardiac conduction negative regulation of TORC1 signaling negative regulation of glycolytic process through fructose-6-phosphate protein localization to lipid particle regulation of bicellular tight junction assembly

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PKA alpha Polyclonal Antibody

PKA alpha Antibody (PA5-143276) in WB

Product Details

PA5-143276

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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