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Invitrogen

PKC alpha Polyclonal Antibody

Advanced Verification
3 Published Figures
4 References
View all (56) PKC alpha antibodies

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Product Details

PA5-17551

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 3 publications 3 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50
-

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to human PKCa

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

11 µg/mL

Amount

1.1 µg

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982440

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with endogenous levels of other PKC isoforms.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AAG6; aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C alpha-polypeptide; protein kinase c-alpha; unnamed protein product

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Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PKCalpha; PKCI+/-; PRKACA; PRKCA

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UniProt ID: (Human) P17252, (Mouse) P20444, (Rat) P05696

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Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity receptor binding integrin binding protein binding ATP binding zinc ion binding kinase activity transferase activity enzyme binding diacylglycerol binding histone kinase activity (H3-T6 specific) metal ion binding protein serine kinase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
response to reactive oxygen species angiogenesis positive regulation of endothelial cell proliferation desmosome assembly chromatin remodeling protein phosphorylation apoptotic process mitotic nuclear envelope disassembly cell adhesion learning or memory response to mechanical stimulus response to toxic substance positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of translation central nervous system neuron axonogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration positive regulation of lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion response to estradiol negative regulation of glial cell apoptotic process intracellular signal transduction proteasome-mediated ubiquitin-dependent protein catabolic process response to peptide hormone regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration response to ethanol positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of bone resorption positive regulation of cell adhesion positive regulation of exocytosis positive regulation of mitotic cell cycle positive regulation of smooth muscle cell proliferation regulation of transport response to corticosterone positive regulation of protein kinase B signaling positive regulation of synapse assembly renal sodium ion absorption positive regulation of ERK1 and ERK2 cascade protein kinase C signaling response to interleukin-1 regulation of platelet aggregation apoptotic signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of angiotensin-activated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response response to phorbol 13-acetate 12-myristate positive regulation of dense core granule biogenesis inactivation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of the force of heart contraction chondrocyte differentiation negative regulation of protein kinase activity cellular calcium ion homeostasis regulation of muscle contraction negative regulation of cell proliferation phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation neutrophil chemotaxis histone H3-T6 phosphorylation peptidyl-serine autophosphorylation negative regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway protein autophosphorylation regulation of receptor-mediated endocytosis positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis cellular response to carbohydrate stimulus intrinsic apoptotic signaling pathway aging response to organic cyclic compound establishment of protein localization response to antibiotic
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