Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
1:50 | - |
Immunoprecipitation (IP) |
1:50 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to human PKCa |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
11 µg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains |
no preservative |
Storage conditions |
-20°C |
RRID |
AB_10982440 |
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with endogenous levels of other PKC isoforms.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha
Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PRKACA; PRKCA
UniProt ID: (Human) P17252, (Mouse) P20444
Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680
Molecular Function:
non-receptor serine/threonine protein kinase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support