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PKC beta Polyclonal Antibody

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Antibody Testing Data (9)

FIGURE: 1 / 9

PKC beta Antibody (12919-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 12919-1-AP) PKC beta antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

PKC beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens).

PKC beta Antibody in Flow Cytometry (Flow) — 1X10^6 K-562 cells were intracellularly stained with 0.2 µg Anti-Human PKC Beta (Product # 12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .

PKC beta Antibody in Immunoprecipitation (IP) — IP result of anti-PKC beta (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

Product Details

12919-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PKC beta Fusion Protein Ag3591 (342-598 aa encoded by BC036472)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: FNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHA (342-598 aa encoded by BC036472)

Target Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Perinatal Fat-Diets Increased Angiotensin II-Mediated Ca²⁺ through PKC-L-Type Calcium Channel Axis in Resistance Arteries via Agtr1a-Prkcb Gene Methylation.

Nutrients

Zheng Q,He Y,Li L,Rui C,Li N,Zhang Y,Ye Y,Zhang Z,Yang X,Tang J,Xu Z

Tue Jan 03 00:00:00 EST 2023

Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.

Acta pharmacologica Sinica

Liu LL,Han Y,Zhang ZJ,Wang YQ,Hu YW,Kaznacheyeva E,Ding JQ,Guo DK,Wang GH,Li B,Ren HG

Thu May 25 00:00:00 EDT 2023

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Journal of hematology & oncology

Xu N,Yao Z,Shang G,Ye D,Wang H,Zhang H,Qu Y,Xu F,Wang Y,Qin Z,Zhu J,Zhang F,Feng J,Tian S,Liu Y,Zhao J,Hou J,Guo J,Hou Y,Ding C

Fri Jun 03 00:00:00 EDT 2022

POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulation HIF1A signaling pathway.

Cell death & disease

Xiao W,Geng W,Zhou M,Xu J,Wang S,Huang Q,Sun Y,Li Y,Yang G,Jin Y

Tue May 03 00:00:00 EDT 2022

Integrating network pharmacology deciphers the action mechanism of Zuojin capsule in suppressing colorectal cancer.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fan JH,Xu MM,Zhou LM,Gui ZW,Huang L,Li XG,Ye XL

Tue Feb 01 00:00:00 EST 2022

PKC signal amplification suppresses non-small cell lung cancer growth by promoting p21 expression and phosphorylation.

Heliyon

Liu S,Zhang Y,Yang Q,Zhang Y,Liu H,Huang MH,Wang R,Lu F

Thu Sep 01 00:00:00 EDT 2022

Prostaglandin E₃ attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Journal of cellular and molecular medicine

Cui J,Shan K,Yang Q,Qi Y,Qu H,Li J,Wang R,Jia L,Chen W,Feng N,Chen YQ

Tue Jun 01 00:00:00 EDT 2021

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Inflammatory bowel diseases

Su X,Wei J,Qi H,Jin M,Zhang Q,Zhang Y,Zhang C,Yang R

Tue Jul 27 00:00:00 EDT 2021

In utero hypoxia altered Ang II-induced contraction via PKCβ in fetal cerebral arteries.

The Journal of endocrinology

Su H,Chen X,Zhang Y,Qi L,He Y,Lv J,Zhang Y,Li X,Tang J,Xu Z

Wed Jan 01 00:00:00 EST 2020

Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling.

American journal of respiratory cell and molecular biology

Snow JB,Norton CE,Sands MA,Weise-Cross L,Yan S,Herbert LM,Sheak JR,Gonzalez Bosc LV,Walker BR,Kanagy NL,Jernigan NL,Resta TC

Mon Jun 01 00:00:00 EDT 2020

PKCβ and reactive oxygen species mediate enhanced pulmonary vasoconstrictor reactivity following chronic hypoxia in neonatal rats.

American journal of physiology. Heart and circulatory physiology

Sheak JR,Yan S,Weise-Cross L,Ahmadian R,Walker BR,Jernigan NL,Resta TC

Sat Feb 01 00:00:00 EST 2020

Prenatal hypoxia attenuated contraction of offspring coronary artery associated with decreased PKCβ Ser⁶⁶⁰ phosphorylation and intracellular calcium.

Life sciences

Chen X,Qi L,Su H,He Y,Li N,Gao Q,Li H,Xu T,Lu L,Xu Z,Tang J

Sun Nov 15 00:00:00 EST 2020

p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.

Oxidative medicine and cellular longevity

Zhang M,Tang J,Shan H,Zhang Q,Yang X,Zhang J,Li Y

Tue Oct 16 00:00:00 EDT 2018

p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.

Oxidative medicine and cellular longevity

Zhang M,Tang J,Shan H,Zhang Q,Yang X,Zhang J,Li Y

Tue Oct 16 00:00:00 EDT 2018

Revealing Synergistic Mechanism of Multiple Components in Gandi Capsule for Diabetic Nephropathy Therapeutics by Network Pharmacology.

Evidence-based complementary and alternative medicine : eCAM

Zhang J,Zhang Q,Chen X,Liu Y,Xue J,Dahan A,Zhang H,Chai Y

Thu Oct 01 00:00:00 EDT 2020

Revealing Synergistic Mechanism of Multiple Components in Gandi Capsule for Diabetic Nephropathy Therapeutics by Network Pharmacology.

Evidence-based complementary and alternative medicine : eCAM

Zhang J,Zhang Q,Chen X,Liu Y,Xue J,Dahan A,Zhang H,Chai Y

Thu Oct 01 00:00:00 EDT 2020

Effect of corticision on orthodontic tooth movement in a rat model as assessed by RNA sequencing.

Journal of molecular histology

Gu Q,Guo S,Wang D,Zhou T,Wang L,Wang Z,Ma J

Thu Jun 01 00:00:00 EDT 2017

Effect of corticision on orthodontic tooth movement in a rat model as assessed by RNA sequencing.

Journal of molecular histology

Gu Q,Guo S,Wang D,Zhou T,Wang L,Wang Z,Ma J

Thu Jun 01 00:00:00 EDT 2017

Gefitinib enhances oxaliplatin-induced apoptosis mediated by Src and PKC-modulated gap junction function.

Oncology reports

Wu JF,Ji J,Dong SY,Li BB,Yu ML,Wu DD,Tao L,Tong XH

Thu Dec 01 00:00:00 EST 2016

Gefitinib enhances oxaliplatin-induced apoptosis mediated by Src and PKC-modulated gap junction function.

Oncology reports

Wu JF,Ji J,Dong SY,Li BB,Yu ML,Wu DD,Tao L,Tong XH

Thu Dec 01 00:00:00 EST 2016

Eupolyphaga sinensis walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling.

Scientific reports

Zhang Y,Zhan Y,Zhang D,Dai B,Ma W,Qi J,Liu R,He L

Tue Jul 01 00:00:00 EDT 2014

Eupolyphaga sinensis walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling.

Scientific reports

Zhang Y,Zhan Y,Zhang D,Dai B,Ma W,Qi J,Liu R,He L

Tue Jul 01 00:00:00 EDT 2014

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway.

Journal of cellular and molecular medicine

Luo W,Wang X,Zheng L,Zhan Y,Zhang D,Zhang J,Zhang Y

Tue Oct 01 00:00:00 EDT 2013

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway.

Journal of cellular and molecular medicine

Luo W,Wang X,Zheng L,Zhan Y,Zhang D,Zhang J,Zhang Y

Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: PKC-B; PKC-beta; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide

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Gene Aliases: A130082F03Rik; PKC-Beta; PKCB; PRKCB; PRKCB1; PRKCB2

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UniProt ID: (Human) P05771, (Mouse) P68404, (Rat) P68403

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Entrez Gene ID: (Human) 5579, (Mouse) 18751, (Rat) 25023

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Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

Function(s)

response to hypoxia adaptive immune response transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis apoptotic process mitotic nuclear envelope disassembly signal transduction negative regulation of glucose transport peptidyl-serine phosphorylation platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation intracellular signal transduction B cell activation lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus immune system process regulation of transcription, DNA-templated regulation of dopamine secretion phosphorylation chromatin modification regulation of growth response to drug response to glucose dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth response to ethanol

Process(es)

chromatin binding protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein kinase C binding calcium channel regulator activity protein binding ATP binding zinc ion binding ligand-dependent nuclear receptor transcription coactivator activity histone kinase activity (H3-T6 specific) histone binding androgen receptor binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PKC beta Polyclonal Antibody

PKC beta Antibody (12919-1-AP) in WB

Product Details

12919-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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