Invitrogen

PKC epsilon Polyclonal Antibody

View all (52) PKC epsilon antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-96060

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human PKC epsilon (NP_0053911)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807862

Product Specific Information

Immunogen sequence: YVNGGDLMFQ IQRSRKFDEP RSRFYAAEVT SALMFLHQHG VIYRDLKLDN ILLDAEGHCK LADFGMCKEG ILNGVTTTTF CGTPDYIAPE ILQELEYGPS VDWWALGVLM YEMMAGQPPF EADNEDDLFE SILHDDVLYP VWLSKEAVSI LKAFMTKNPH KRLGCVASQN GEDAIKQHPF FKEIDWVLLE QKKIKPPFKP RIKTKRDVNN FDQDFTREEP VLTLVDEAIV KQINQEEFKG FSYFGEDLMP; Positive Samples: 293T, HT-29, Mouse brain, Mouse lung; Cellular Location: Cell membrane, Cytoplasm, Nucleus, cytoskeleton, perinuclear region

Target Information

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

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Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

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UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

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Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

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Function(s)
actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration
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