Invitrogen

PKC gamma Polyclonal Antibody

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PKC gamma Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of PKC-gamma was performed in frozen-sectioned adult mouse cerebellum tissue using PKC gamma Polyclonal Antibody (Product # PA5-27732) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

PKC gamma Antibody in Western Blot (WB) — Western Blot analysis of PKC gamma was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC gamma Polyclonal Antibody (Product # PA5-27732) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-27732

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 1 and 166 of Human PKC gamma View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.55 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545208

Product Specific Information

Recommended positive controls: Molt-4, mouse brain, rat brain.

Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (98%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.