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Invitrogen
PKC lambda/iota Polyclonal Antibody
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Product Details
PA5-79872
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PKC iota recombinant protein (Position: D21-Q214).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: SHG whole cell, A549 whole cell, U87 whole cell, 293T whole cell, HELA whole cell, JURKAT whole cell. IHC: Mouse Brain Tissue, Rat Brain Tissue, Human Lung Cancer Tissue.
Target Information
The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aPKC-lambda/iota; Atypical protein kinase C-lambda/iota; nPKC-iota; PRKC-lambda/iota; Protein kinase C iota type; protein kinase C, lambda; unnamed protein product
Gene Aliases: 2310021H13Rik; AI427505; aPKClambda; DXS1179E; mKIAA4165; nPKC-iota; PKCI; Pkcl; PKClambda; PRKCI; Prkcl
UniProt ID: (Human) P41743, (Mouse) Q62074, (Rat) F1M7Y5
Entrez Gene ID: (Human) 5584, (Mouse) 18759, (Rat) 84006
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
protein binding
ATP binding
phospholipid binding
zinc ion binding
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
protein domain specific binding
molecular_function
non-receptor serine/threonine protein kinase
protein phosphorylation
protein targeting to membrane
cytoskeleton organization
actin filament organization
positive regulation of neuron projection development
vesicle-mediated transport
cell migration
establishment of cell polarity
cellular response to insulin stimulus
negative regulation of glial cell apoptotic process
establishment of apical/basal cell polarity
intracellular signal transduction
eye photoreceptor cell development
negative regulation of apoptotic process
response to peptide hormone
negative regulation of neuron apoptotic process
establishment or maintenance of epithelial cell apical/basal polarity
cell-cell junction organization
positive regulation of Notch signaling pathway
positive regulation of glucose import
secretion
Golgi vesicle budding
positive regulation of NF-kappaB transcription factor activity
positive regulation of glial cell proliferation
membrane organization
cellular response to chemical stress
response to interleukin-1
protein localization to plasma membrane
regulation of postsynaptic membrane neurotransmitter receptor levels
positive regulation of protein localization to plasma membrane
positive regulation of endothelial cell apoptotic process
multicellular organism development
phosphorylation
cellular protein localization
positive regulation of establishment of protein localization to plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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