Invitrogen

PKC lambda/iota Monoclonal Antibody (F.699.5)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (14) PKC lambda/iota antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PKC lambda/iota Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PRKCI was performed using C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRKCI Rabbit Monoclonal Antibody (Product # MA5-14874) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing cytoplasmic staining of PRKCI in C2C12 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PKC lambda/iota Antibody in Western Blot (WB) — Western blot was performed using Anti-PRKCI Antibody (Product # MA5-14874) and a 78 kDa band corresponding to PRKCI was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of PC-12 (Lane 1), C2C12 (Lane 2), Mouse Lung (Lane 3) and Rat Lung (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

PKC lambda/iota Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. C2C12 cells were transfected with PRKCI siRNA and decrease in signal intensity was observed in western blot application using Anti- PRKCI Monoclonal Antibody (Product # MA5-14874). {KD}

Product Details

MA5-14874

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	F.699.5
Immunogen	Synthetic peptide corresponding to human PKC lambda/iota
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10986838

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other PKC isoforms.

Target Information

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aPKC-lambda/iota; Atypical protein kinase C-lambda/iota; nPKC-iota; PRKC-lambda/iota; Protein kinase C iota type; protein kinase C, iota; protein kinase C, lambda

Gene Aliases: 2310021H13Rik; AI427505; aPKClambda; DXS1179E; mKIAA4165; nPKC-iota; PKCI; Pkcl; PKClambda; PRKCI; Prkcl

UniProt ID: (Human) P41743, (Mouse) Q62074, (Rat) F1M7Y5

Entrez Gene ID: (Human) 5584, (Mouse) 18759, (Rat) 84006

Molecular Function: non-receptor serine/threonine protein kinase