Invitrogen

PKD2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (14) PKD2 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

PKD2 Antibody in Immunocytochemistry (ICC/IF) — Published figure using PKD2 polyclonal antibody (Product # OSP00017W) in Immunocytochemistry

PKD2 Antibody in Western Blot (WB) — Published figure using PKD2 polyclonal antibody (Product # OSP00017W) in Western Blot

PKD2 Antibody — Figure 1. Onset of kidney tubule cyst formation in Arl13b-EGFPtg:cPkd1 and Arl13b-EGFPtg:cPkd2 animals. (A) Study design to assess cyst formation after genetic ablation of either Pkd1 or Pkd2. One protocol is shown that assesses polycystin-1 or polycystin-2 at P28 in the conditional knockout mouse. (B) Loss of polycystin-1 and polycystin-2 protein expression as assessed by immunoblot, 2 weeks and 2 months after doxycycline removal. Whole cell lysates were prepared from pIMCD cells and subjected to western blot analysis (three independent experiments). A trace amount of polycystin-2 (first row, faint band at 2 weeks after doxycycline removal) is similar to that previously reported (Ma et al., 2013). The band at the left edge (cPkd1, 2 weeks + Doxycycline, first row), appears to be nonspecific, although we cannot rule out polycystin-1 contamination from non-tubule cells. (C) Kidney weight/body was increased in Arl13b-EGFPtg:cPkd2 mice with doxycycline treatment compared to control littermates without doxycycline treatment. (D) Cystic index (Materials and methods) shows that cysts increased in Arl13b-EGFPtg:cPkd2 mice. (E) Kidney weight/body was increased in Arl13b-EGFPtg:cPkd1 with doxycycline treatment (2 months after doxycycline removal) compared to control littermates without doxycycline treatment. (F) Cystic index shows increased size and number of cysts in Arl13b-EGFPtg:cPkd1 mice.

Product Details

OSP00017W

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:300-1:2,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from mouse PKD2 conjugated to blue carrier protein was used as the antigen.

Conjugate

Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

RRID

AB_2163366

Product Specific Information

This antibody is directed against the unphosphorylated form of PKD2, and the cross-reactivity with phosphorylated forms has not yet been determined.

Reconstitute in 100 µL of sterile water. When reconstituting, glycerol (1:1) may be added for an additional stability.

Target Information

This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polycystin-2 is an essential ion channel subunit in the primary cilium of the renal collecting duct epithelium.

eLife

Liu X,Vien T,Duan J,Sheu SH,DeCaen PG,Clapham DE

Published figure using PKD2 polyclonal antibody (Product # OSP00017W) in Immunofluorescence

Wed Feb 14 00:00:00 EST 2018