Proteintech

PKLR Polyclonal Antibody

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PKLR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 40x lens).

PKLR Antibody in Western Blot (WB) — WB results of PKM1, PKM2, PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver.).

Product Details

22456-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PKLR Fusion Protein Ag17933 (446-556 aa encoded by BC025737)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: PLSRDPTEV TAIGAVEAAF KCCAAAIIVL TTTGRSAQLL SRYRPRAAVI AVTRSAQAAR QVHLCRGVFP LLYREPPEAI WADDVDRRVQ FGIESGKLRG FLRVGDLVIV VT (446-556 aa encoded by BC025737)

Target Information

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis.

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Wed Feb 02 00:00:00 EST 2022

NLRP3 inflammasome blocked the glycolytic pathway via targeting to PKLR in arsenic-induced hepatic insulin resistance.

Ecotoxicology and environmental safety

Zhang J,Qiu T,Jiang L,Wang N,Zhu Y,Yan R,Wang S,Bai J,Shi X,Yang G,Liu X,Yao X,Sun X

Fri Oct 15 00:00:00 EDT 2021

Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.

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Wed Feb 10 00:00:00 EST 2021

Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice.

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Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.

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Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.

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The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

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The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

Yang Y,Zhu G,Dong B,Piao J,Chen L,Lin Z

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The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

Yang Y,Zhu G,Dong B,Piao J,Chen L,Lin Z

Mon Jul 01 00:00:00 EDT 2019

The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

Yang Y,Zhu G,Dong B,Piao J,Chen L,Lin Z

Mon Jul 01 00:00:00 EDT 2019

Role of AMPD2 in impaired glucose tolerance induced by high fructose diet.

Molecular genetics and metabolism reports

Hudoyo AW,Hirase T,Tandelillin A,Honda M,Shirai M,Cheng J,Morisaki H,Morisaki T

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Role of AMPD2 in impaired glucose tolerance induced by high fructose diet.

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Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

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Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

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Thu Jun 01 00:00:00 EDT 2017

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

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Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Retinoic acid-related orphan receptor γ (RORγ): a novel participant in the diurnal regulation of hepatic gluconeogenesis and insulin sensitivity.

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Mon Dec 15 00:00:00 EST 2014

Retinoic acid-related orphan receptor γ (RORγ): a novel participant in the diurnal regulation of hepatic gluconeogenesis and insulin sensitivity.

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Bioinformatics

Protein Aliases: isozymes R/L; L-PK; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; Pyruvate kinase PKLR; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and red blood cell; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase

Gene Aliases: Pk-1; PK1; PKL; Pklg; PKLR; PKR; PKRL; R-PK; RPK

UniProt ID: (Human) P30613, (Rat) P12928

Entrez Gene ID: (Human) 5313, (Mouse) 18770, (Rat) 24651

Molecular Function: kinase