Proteintech

PKM Polyclonal Antibody

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PKM Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10078-2-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PKM Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 40x lens).

PKM Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

10078-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PKM Fusion Protein Ag0110 (11-221 aa encoded by BC000481) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.14 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can recognize both PK-M1 and PK-M2.

Immunogen sequence: AFIQTQQLH AAMADTFLEH MCRLDIDSPP ITARNTGIIC TIGPASRSVE TLKEMIKSGM NVARLNFSHG THEYHAETIK NVRTATESFA SDPILYRPVA VALDTKGPEI RTGLIKGSGT AEVELKKGAT LKITLDNAYM EKCDENILWL DYKNICKVVE VGSKIYVDDG LISLQVKQKG ADFLVTEVEN GGSLGSKKGV NLPGAAVDLP AV (11-221 aa encoded by BC000481)

Target Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points.

Journal of pain research

Li LH,Huang QM,Barbero M,Liu L,Nguyen TT,Beretta-Piccoli M,Xu AL,Ji LJ

Thu Mar 31 00:00:00 EDT 2022

The combination of metformin and 2-deoxyglucose significantly inhibits cyst formation in miniature pigs with polycystic kidney disease.

British journal of pharmacology

Lian X,Wu X,Li Z,Zhang Y,Song K,Cai G,Li Q,Lin S,Chen X,Bai XY

Fri Mar 01 00:00:00 EST 2019

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 EST 2017

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Scientific reports

Fu L,Xu Y,Hou Y,Qi X,Zhou L,Liu H,Luan Y,Jing L,Miao Y,Zhao S,Liu H,Li X

Mon Mar 27 00:00:00 EDT 2017

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

The Analyst

Yu Y,Pan X,Ding Y,Liu X,Tang H,Shen C,Shen H,Yang P

Wed Aug 21 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK; Tyrosine-protein kinase PKM2

Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM2; Pykm; TCB; THBP1

UniProt ID: (Human) P14618, (Mouse) P52480

Entrez Gene ID: (Human) 5315, (Mouse) 18746

Molecular Function: kinase