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PKM Polyclonal Antibody

PKM Antibody in Immunocytochemistry (ICC/IF)

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PKM Antibody (10078-2-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10078-2-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PKM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

10078-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PKM Fusion Protein Ag0110 (11-221 aa encoded by BC000481)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize both PK-M1 and PK-M2.

Immunogen sequence: AFIQTQQLH AAMADTFLEH MCRLDIDSPP ITARNTGIIC TIGPASRSVE TLKEMIKSGM NVARLNFSHG THEYHAETIK NVRTATESFA SDPILYRPVA VALDTKGPEI RTGLIKGSGT AEVELKKGAT LKITLDNAYM EKCDENILWL DYKNICKVVE VGSKIYVDDG LISLQVKQKG ADFLVTEVEN GGSLGSKKGV NLPGAAVDLP AV (11-221 aa encoded by BC000481)

Target Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Fri Apr 15 00:00:00 UTC 2022

Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.

American journal of translational research

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Tue Dec 28 00:00:00 UTC 2021

Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.

American journal of translational research

Wen LJ,Hu XL,Li CY,Liu J,Li ZY,Li YZ,Zhou JY

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Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.

American journal of translational research

Wen LJ,Hu XL,Li CY,Liu J,Li ZY,Li YZ,Zhou JY

Tue Dec 28 00:00:00 UTC 2021

Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.

American journal of translational research

Wen LJ,Hu XL,Li CY,Liu J,Li ZY,Li YZ,Zhou JY

Tue Dec 28 00:00:00 UTC 2021

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Science advances

Asamitsu S,Yabuki Y,Ikenoshita S,Kawakubo K,Kawasaki M,Usuki S,Nakayama Y,Adachi K,Kugoh H,Ishii K,Matsuura T,Nanba E,Sugiyama H,Fukunaga K,Shioda N

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Glycolytic and lipid oxidative metabolic programs are essential for freshly-isolated regulatory T cells in mice with sepsis.

RSC advances

Zhu X,Ji W,Guo S,Zhu D,Yang Y,Liu X

Wed May 27 00:00:00 UTC 2020

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

OncoTargets and therapy

Dai J,Jiang L,Qiu L,Shao Y,Shi P,Li J

Sat Apr 16 00:00:00 UTC 2022

The combination of metformin and 2-deoxyglucose significantly inhibits cyst formation in miniature pigs with polycystic kidney disease.

British journal of pharmacology

Lian X,Wu X,Li Z,Zhang Y,Song K,Cai G,Li Q,Lin S,Chen X,Bai XY

Fri Mar 01 00:00:00 UTC 2019

The combination of metformin and 2-deoxyglucose significantly inhibits cyst formation in miniature pigs with polycystic kidney disease.

British journal of pharmacology

Lian X,Wu X,Li Z,Zhang Y,Song K,Cai G,Li Q,Lin S,Chen X,Bai XY

Fri Mar 01 00:00:00 UTC 2019

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Journal of pain research

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Thu Mar 31 00:00:00 UTC 2022

Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points.

Journal of pain research

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Thu Mar 31 00:00:00 UTC 2022

Pyridostigmine regulates glucose metabolism and mitochondrial homeostasis to reduce myocardial vulnerability to injury in diabetic mice.

American journal of physiology. Endocrinology and metabolism

Yang Y,Zhao M,Yu XJ,Liu LZ,He X,Deng J,Zang WJ

Thu Aug 01 00:00:00 UTC 2019

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Scientific reports

Fu L,Xu Y,Hou Y,Qi X,Zhou L,Liu H,Luan Y,Jing L,Miao Y,Zhao S,Liu H,Li X

Mon Mar 27 00:00:00 UTC 2017

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Scientific reports

Fu L,Xu Y,Hou Y,Qi X,Zhou L,Liu H,Luan Y,Jing L,Miao Y,Zhao S,Liu H,Li X

Mon Mar 27 00:00:00 UTC 2017

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 UTC 2017

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

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The Analyst

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An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

The Analyst

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Bioinformatics

Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK; Tyrosine-protein kinase PKM2

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Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM2; Pykm; TCB; THBP1

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UniProt ID: (Human) P14618, (Mouse) Q64484

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Entrez Gene ID: (Human) 5315, (Mouse) 18746

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Function(s)

magnesium ion binding pyruvate kinase activity protein binding ATP binding kinase activity MHC class II protein complex binding potassium ion binding ADP binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity transferase activity metal ion binding kinase

Process(es)

response to hypoxia liver development ATP biosynthetic process response to nutrient response to gravity programmed cell death response to muscle inactivity organ regeneration response to insulin pyruvate biosynthetic process skeletal muscle tissue regeneration canonical glycolysis cell-cell adhesion glucose metabolic process glycolytic process metabolic process phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PKM Polyclonal Antibody

PKM Antibody (10078-2-AP) in ICC/IF

Product Details

10078-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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