Proteintech

PKM1 Polyclonal Antibody

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PKM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

PKM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 15821-1-AP ( PKM1-specific antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PKM1 Antibody in Western Blot (WB) — WB results of PKM1, PKM2, PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver.).

PKM1 Antibody in Immunoprecipitation (IP) — IP result of anti-PKM1-specific (IP:15821-1-AP, 3ug; Detection:15821-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

15821-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 22 publications 22 publications
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Artificial Control, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.45 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to PKM1 isoform.

Target Information

PKM1 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway.

Frontiers in pharmacology

Jiang H,Zhang S,Song T,Guan X,Zhang R,Chen X

Tue Sep 29 00:00:00 EDT 2020

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

PloS one

Ma B,Chen J,Mu Y,Xue B,Zhao A,Wang D,Chang D,Pan Y,Liu J

Mon Feb 11 00:00:00 EST 2019

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Physiological genomics

Williams AL,Khadka V,Tang M,Avelar A,Schunke KJ,Menor M,Shohet RV

Sun Jul 01 00:00:00 EDT 2018

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.

Nucleic acids research

Hilander T,Zhou XL,Konovalova S,Zhang FP,Euro L,Chilov D,Poutanen M,Chihade J,Wang ED,Tyynismaa H

Thu Jan 25 00:00:00 EST 2018

Pkm2 can enhance pluripotency in ESCs and promote somatic cell reprogramming to iPSCs.

Oncotarget

Qin S,Yang D,Chen K,Li H,Zhang L,Li Y,Le R,Li X,Gao S,Kang L

Fri Oct 13 00:00:00 EDT 2017

Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells.

Cellular signalling

Horiuchi Y,Nakatsu D,Kano F,Murata M

Sun Oct 01 00:00:00 EDT 2017

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression.

Cell death & disease

Wei L,Dai Y,Zhou Y,He Z,Yao J,Zhao L,Guo Q,Yang L

Thu Jul 20 00:00:00 EDT 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 EDT 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 EDT 2017

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

Nature communications

Wei Y,Wang D,Jin F,Bian Z,Li L,Liang H,Li M,Shi L,Pan C,Zhu D,Chen X,Hu G,Liu Y,Zhang CY,Zen K

Mon Jan 09 00:00:00 EST 2017

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Biochemical and biophysical research communications

Dong K,Ni H,Wu M,Tang Z,Halim M,Shi D

Fri Aug 05 00:00:00 EDT 2016

Alterations in glucose metabolism proteins responsible for the Warburg effect in esophageal squamous cell carcinoma.

Experimental and molecular pathology

de Andrade Barreto E,de Souza Santos PT,Bergmann A,de Oliveira IM,Wernersbach Pinto L,Blanco T,Rossini A,Pinto Kruel CD,Mattos Albano R,Ribeiro Pinto LF

Mon Aug 01 00:00:00 EDT 2016

PKM2 uses control of HuR localization to regulate p27 and cell cycle progression in human glioblastoma cells.

International journal of cancer

Mukherjee J,Ohba S,See WL,Phillips JJ,Molinaro AM,Pieper RO

Fri Jul 01 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.

PloS one

Konno M,Koseki J,Kawamoto K,Nishida N,Matsui H,Dewi DL,Ozaki M,Noguchi Y,Mimori K,Gotoh N,Tanuma N,Shima H,Doki Y,Mori M,Ishii H

Mon Apr 25 00:00:00 EDT 2016

M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.

Investigative ophthalmology & visual science

Casson RJ,Wood JP,Han G,Kittipassorn T,Peet DJ,Chidlow G

Fri Jan 01 00:00:00 EST 2016

M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.

Investigative ophthalmology & visual science

Casson RJ,Wood JP,Han G,Kittipassorn T,Peet DJ,Chidlow G

Fri Jan 01 00:00:00 EST 2016

Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.

Biochimica et biophysica acta

Santi A,Caselli A,Ranaldi F,Paoli P,Mugnaioni C,Michelucci E,Cirri P

Tue Dec 01 00:00:00 EST 2015

A small subunit processome protein promotes cancer by altering translation.

Oncogene

Yang HW,Kim TM,Song SS,Menon L,Jiang X,Huang W,Black PM,Park PJ,Carroll RS,Johnson MD

Thu Aug 20 00:00:00 EDT 2015

L-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2.

Proceedings of the National Academy of Sciences of the United States of America

Nakatsu D,Horiuchi Y,Kano F,Noguchi Y,Sugawara T,Takamoto I,Kubota N,Kadowaki T,Murata M

Tue Mar 10 00:00:00 EDT 2015

Estradiol-17β upregulates pyruvate kinase M2 expression to coactivate estrogen receptor-α and to integrate metabolic reprogramming with the mitogenic response in endometrial cells.

The Journal of clinical endocrinology and metabolism

Salama SA,Mohammad MA,Diaz-Arrastia CR,Kamel MW,Kilic GS,Ndofor BT,Abdel-Baki MS,Theiler SK

Wed Oct 01 00:00:00 EDT 2014

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

PloS one

Liu S,Miriyala S,Keaton MA,Jordan CT,Wiedl C,Clair DK,Moscow JA

Sun Sep 21 00:00:00 EDT 2014

The lack of evidence for correlation of pyruvate kinase M2 expression with tumor grade in non-small cell lung cancer.

Anticancer research

Kobierzycki C,Pula B,Werynska B,Piotrowska A,Muszczynska-Bernhard B,Dziegiel P,Rakus D

Tue Jul 01 00:00:00 EDT 2014

Embelin inhibits endothelial mitochondrial respiration and impairs neoangiogenesis during tumor growth and wound healing.

EMBO molecular medicine

Coutelle O,Hornig-Do HT,Witt A,Andree M,Schiffmann LM,Piekarek M,Brinkmann K,Seeger JM,Liwschitz M,Miwa S,Hallek M,Krönke M,Trifunovic A,Eming SA,Wiesner RJ,Hacker UT,Kashkar H

Thu May 01 00:00:00 EDT 2014

Pyruvate kinase M2 expression, but not pyruvate kinase activity, is up-regulated in a grade-specific manner in human glioma.

PloS one

Mukherjee J,Phillips JJ,Zheng S,Wiencke J,Ronen SM,Pieper RO

Tue Jan 21 00:00:00 EST 2014

Pyruvate kinase M2 expression, but not pyruvate kinase activity, is up-regulated in a grade-specific manner in human glioma.

PloS one

Mukherjee J,Phillips JJ,Zheng S,Wiencke J,Ronen SM,Pieper RO

Tue Jan 21 00:00:00 EST 2014

Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?

Seminars in oncology

Martinez-Outschoorn UE,Whitaker-Menezes D,Valsecchi M,Martinez-Cantarin MP,Dulau-Florea A,Gong J,Howell A,Flomenberg N,Pestell RG,Wagner J,Arana-Yi C,Sharma M,Sotgia F,Lisanti MP

Thu Aug 01 00:00:00 EDT 2013

Manipulation of PK-M mutually exclusive alternative splicing by antisense oligonucleotides.

Open biology

Wang Z,Jeon HY,Rigo F,Bennett CF,Krainer AR

Mon Oct 01 00:00:00 EDT 2012

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

Cancer biology & therapy

Chiavarina B,Whitaker-Menezes D,Martinez-Outschoorn UE,Witkiewicz AK,Birbe R,Howell A,Pestell RG,Smith J,Daniel R,Sotgia F,Lisanti MP

Thu Dec 15 00:00:00 EST 2011

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

Cancer biology & therapy

Chiavarina B,Whitaker-Menezes D,Martinez-Outschoorn UE,Witkiewicz AK,Birbe R,Howell A,Pestell RG,Smith J,Daniel R,Sotgia F,Lisanti MP

Thu Dec 15 00:00:00 EST 2011

Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells.

Cell cycle (Georgetown, Tex.)

Martinez-Outschoorn UE,Balliet RM,Rivadeneira DB,Chiavarina B,Pavlides S,Wang C,Whitaker-Menezes D,Daumer KM,Lin Z,Witkiewicz AK,Flomenberg N,Howell A,Pestell RG,Knudsen ES,Sotgia F,Lisanti MP

Sun Aug 15 00:00:00 EDT 2010

The reverse Warburg effect: glycolysis inhibitors prevent the tumor promoting effects of caveolin-1 deficient cancer associated fibroblasts.

Cell cycle (Georgetown, Tex.)

Bonuccelli G,Whitaker-Menezes D,Castello-Cros R,Pavlides S,Pestell RG,Fatatis A,Witkiewicz AK,Vander Heiden MG,Migneco G,Chiavarina B,Frank PG,Capozza F,Flomenberg N,Martinez-Outschoorn UE,Sotgia F,Lisanti MP

Sat May 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; PKM1; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK; Tyrosine-protein kinase PKM2

Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM2; Pykm; TCB; THBP1

UniProt ID: (Human) P14618, (Mouse) P52480

Entrez Gene ID: (Human) 5315, (Mouse) 18746

Molecular Function: kinase

Function(s)

response to hypoxia liver development ATP biosynthetic process response to nutrient response to gravity programmed cell death response to muscle inactivity organ regeneration response to insulin pyruvate biosynthetic process skeletal muscle tissue regeneration canonical glycolysis cell-cell adhesion glucose metabolic process glycolytic process metabolic process phosphorylation

Process(es)

magnesium ion binding pyruvate kinase activity protein binding ATP binding kinase activity MHC class II protein complex binding potassium ion binding ADP binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity transferase activity metal ion binding

PKM1 Polyclonal Antibody

Product Details

15821-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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