Proteintech

PKM2 Polyclonal Antibody

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PKM2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using PKM2 antibody 19060-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PKM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15822-1-AP ( PKM2-specific antibody at dilution of 1:200 (under 40x lens).

PKM2 Antibody in Western Blot (WB) — WB results of PKM1, PKM2, PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver.).

PKM2 Antibody in Immunoprecipitation (IP) — IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:300) with MCF-7 cells lysate 3250ug.

Product Details

15822-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 27 publications 27 publications
Immunohistochemistry (IHC)	-	View 11 publications 11 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.39 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to PKM2 isoform.

Target Information

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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American journal of cancer research

Wu H,Li Z,Wang Y,Yang P,Li Z,Li H,Wu C

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MiR-106b-mediated Mfn2 suppression is critical for PKM2 induced mitochondrial fusion.

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Wu H,Li Z,Wang Y,Yang P,Li Z,Li H,Wu C

Thu Oct 01 00:00:00 EDT 2020

EGFRwt/vIII-PKM2-β-catenin cascade affects proliferation and chemo-sensitivity in head and neck squamous cell carcinoma.

American journal of cancer research

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Wed Sep 30 00:00:00 EDT 2020

EGFRwt/vIII-PKM2-β-catenin cascade affects proliferation and chemo-sensitivity in head and neck squamous cell carcinoma.

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Wed Sep 30 00:00:00 EDT 2020

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Liu B,Jin J,Zhang Z,Zuo L,Jiang M,Xie C

Thu Aug 01 00:00:00 EDT 2019

Oncoprotein HBXIP induces PKM2 via transcription factor E2F1 to promote cell proliferation in ER-positive breast cancer.

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Liu BW,Wang TJ,Li LL,Zhang L,Liu YX,Feng JY,Wu Y,Xu FF,Zhang QS,Bao MZ,Zhang WY,Ye LH

Mon Apr 01 00:00:00 EDT 2019

Oncoprotein HBXIP induces PKM2 via transcription factor E2F1 to promote cell proliferation in ER-positive breast cancer.

Acta pharmacologica Sinica

Liu BW,Wang TJ,Li LL,Zhang L,Liu YX,Feng JY,Wu Y,Xu FF,Zhang QS,Bao MZ,Zhang WY,Ye LH

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GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.

Cell death & disease

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MiR-139-5p is associated with poor prognosis and regulates glycolysis by repressing PKM2 in gallbladder carcinoma.

Cell proliferation

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Thu Nov 01 00:00:00 EDT 2018

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18F-fluorodeoxyglucose uptake predicts PKM2 expression in lung adenocarcinoma.

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Tue Jun 13 00:00:00 EDT 2017

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

Scientific reports

Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

Fri Jun 10 00:00:00 EDT 2016

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.

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Lu W,Cao Y,Zhang Y,Li S,Gao J,Wang XA,Mu J,Hu YP,Jiang L,Dong P,Gong W,Liu Y

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The lack of evidence for correlation of pyruvate kinase M2 expression with tumor grade in non-small cell lung cancer.

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Thu Aug 01 00:00:00 EDT 2013

A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.

Medical oncology (Northwood, London, England)

Yuan Y,Guo-Qing P,Yan T,Hong-Lin Y,Gong-Hua H,Cai-Gao Z

Sat Dec 01 00:00:00 EST 2012

A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.

Medical oncology (Northwood, London, England)

Yuan Y,Guo-Qing P,Yan T,Hong-Lin Y,Gong-Hua H,Cai-Gao Z

Sat Dec 01 00:00:00 EST 2012

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

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Chiavarina B,Whitaker-Menezes D,Martinez-Outschoorn UE,Witkiewicz AK,Birbe R,Howell A,Pestell RG,Smith J,Daniel R,Sotgia F,Lisanti MP

Thu Dec 15 00:00:00 EST 2011

Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.

Cancer biology & therapy

Chiavarina B,Whitaker-Menezes D,Martinez-Outschoorn UE,Witkiewicz AK,Birbe R,Howell A,Pestell RG,Smith J,Daniel R,Sotgia F,Lisanti MP

Thu Dec 15 00:00:00 EST 2011

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Cell cycle (Georgetown, Tex.)

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Sun Aug 15 00:00:00 EDT 2010

Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells.

Cell cycle (Georgetown, Tex.)

Martinez-Outschoorn UE,Balliet RM,Rivadeneira DB,Chiavarina B,Pavlides S,Wang C,Whitaker-Menezes D,Daumer KM,Lin Z,Witkiewicz AK,Flomenberg N,Howell A,Pestell RG,Knudsen ES,Sotgia F,Lisanti MP

Sun Aug 15 00:00:00 EDT 2010

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Cell cycle (Georgetown, Tex.)

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Cell cycle (Georgetown, Tex.)

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Sat May 15 00:00:00 EDT 2010

The reverse Warburg effect: glycolysis inhibitors prevent the tumor promoting effects of caveolin-1 deficient cancer associated fibroblasts.

Cell cycle (Georgetown, Tex.)

Sat May 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2

Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM2; Pykm; TCB; THBP1

UniProt ID: (Human) P14618, (Mouse) P52480

Entrez Gene ID: (Human) 5315, (Mouse) 18746

Molecular Function: kinase

Function(s)

response to hypoxia liver development ATP biosynthetic process response to nutrient response to gravity programmed cell death response to muscle inactivity organ regeneration response to insulin pyruvate biosynthetic process skeletal muscle tissue regeneration canonical glycolysis cell-cell adhesion glucose metabolic process glycolytic process metabolic process phosphorylation

Process(es)

magnesium ion binding pyruvate kinase activity protein binding ATP binding kinase activity MHC class II protein complex binding potassium ion binding ADP binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity transferase activity metal ion binding

PKM2 Polyclonal Antibody

Product Details

15822-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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