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Invitrogen
PKM2 Polyclonal Antibody
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FIGURE: 1 / 12
PKM2 Antibody (PA5-28700) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PKM2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 Polyclonal Antibody (Product # PA5-28700) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-28700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.13 mg/mL
Amount
13 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain.
Predicted reactivity: Mouse (97%), Rat (88%), Xenopus laevis (90%), Rabbit (97%), Chicken (83%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; cytosolic thyroid hormone-binding protein (EC 2.7.1.40); epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; similar to human pyruvate kinase M2; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2; unnamed protein product
Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; p58; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1
UniProt ID: (Human) P14618, (Mouse) P52480, (Rat) P11980
Entrez Gene ID: (Human) 5315, (Mouse) 18746, (Rat) 25630
nucleotide binding
magnesium ion binding
transcription coactivator activity
RNA binding
mRNA binding
catalytic activity
protein tyrosine kinase activity
pyruvate kinase activity
protein binding
ATP binding
kinase activity
transferase activity
MHC class II protein complex binding
potassium ion binding
histone kinase activity (H3-T11 specific)
protein homodimerization activity
cadherin binding
metal ion binding
ADP binding
poly(A) RNA binding
kinase
glycolytic process
chromatin remodeling
regulation of translation
programmed cell death
cellular response to insulin stimulus
positive regulation of transcription from RNA polymerase II promoter
canonical glycolysis
positive regulation of sprouting angiogenesis
positive regulation of cytoplasmic translation
liver development
glucose metabolic process
ATP biosynthetic process
metabolic process
phosphorylation
organ regeneration
pyruvate biosynthetic process
skeletal muscle tissue regeneration
response to hypoxia
response to nutrient
response to gravity
response to organic substance
response to muscle inactivity
response to insulin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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