Immunofluorescent analysis of PLA2G4F showing staining in the cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PLA2G4F polyclonal antibody (Product # PA5-31736) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 333 and 678 of Human PLA2G4F|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31736 targets PLA2G4F in WB applications and shows reactivity with Human samples.
The PA5-31736 immunogen is recombinant fragment corresponding to a region within amino acids 333 and 678 of Human PLA2G4F.
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.