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Abnova
PLAT Polyclonal Antibody, MaxPab™
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FIGURE: 1 / 1
PLAT Antibody (H00005327-D01P) in WB
Western Blot analysis of PLAT expression in transfected 293T cell line (H00005327-T01) by PLAT MaxPab polyclonal antibody.
Lane 1: PLAT transfected lysate(62.90 KDa).
Lane 2: Non-transfected lysate.
Lane 1: PLAT transfected lysate(62.90 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00005327-D01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PLAT (NP_000921.1, 1 a.a. approximately 562 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MDAMKRGLCC VLLLCGAVFV SPSQEIHARF RRGARSYQVI CRDEKTQMIY QQHQSWLRPV LRSNRVEYCW CNSGRAQCHS VPVKSCSEPR CFNGGTCQQA LYFSDFVCQC PEGFAGKCCE IDTRATCYED QGISYRGTWS TAESGAECTN WNSSALAQKP YSGRRPDAIR LGLGNHNYCR NPDRDSKPWC YVFKAGKYSS EFCSTPACSE GNSDCYFGNG SAYRGTHSLT ESGASCLPWN SMILIGKVYT AQNPSAQALG LGKHNYCRNP DGDAKPWCHV LKNRRLTWEY CDVPSCSTCG LRQYSQPQFR IKGGLFADIA SHPWQAAIFA KHRRSPGERF LCGGILISSC WILSAAHCFQ ERFPPHHLTV ILGRTYRVVP GEEEQKFEVE KYIVHKEFDD DTYDNDIALL QLKSDSSRCA QESSVVRTVC LPPADLQLPD WTECELSGYG KHEALSPFYS ERLKEAHVRL YPSSRCTSQH LLNRTVTDNM LCAGDTRSGG PQANLHDACQ GDSGGPLVCL NDGRMTLVGI ISWGLGCGQK DVPGVYTKVT NYLDWIRDNM RP
Target Information
TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.4.21.68; alteplase; plasminogen activator; plasminogen/activator kringle; reteplase; t plasminogen activator; t-PA; t-plasminogen activator; Tissue-type plasminogen activator; unnamed protein product
Gene Aliases: PLAT; T-PA; TPA
UniProt ID: (Human) P00750
Entrez Gene ID: (Human) 5327
response to hypoxia
proteolysis
blood coagulation
negative regulation of plasminogen activation
smooth muscle cell migration
zymogen activation
plasminogen activation
protein modification process
fibrinolysis
negative regulation of proteolysis
platelet-derived growth factor receptor signaling pathway
negative regulation of fibrinolysis
prevention of polyspermy
trans-synaptic signaling by BDNF, modulating synaptic transmission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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