Invitrogen

PLCB2 Polyclonal Antibody

View all (9) PLCB2 antibodies

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PLCB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PLCB2 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PLCB2 Polyclonal Antibody (Product # PA5-102006) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cell membrane and cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification. THP-1 is high expressing than SK-O-V3 for PLCB2.

PLCB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PLCB2 Polyclonal Antibody (Product # PA5-102006) and a 134 kDa band corresponding to PLCB2 was observed across cell lines. Whole cell extracts (60 µg lysate) of THP-1 (Lane 1), HeLa (Lane 2), HEL 92.1.7 (Lane 3), SK-O-V3 (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). HeLa and SK-O-V3 are expected to be low expressing cell models for PLCB2 than THP-1 and HEL 92.1.7, as observed.

Product Details

PA5-102006

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PLCB2(Accession Q00722), corresponding to amino acid residues S351-T379. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851438

Target Information

PLCB2 is the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2; Phosphoinositide phospholipase C-beta-2; phospholipase C, beta 2; Phospholipase C-beta-2; PKC beta II; PLC beta-2; PLC-beta-2; PLC-beta2; plcb2 {ECO:0000250|UniProtKB:Q00722}; PLCbeta-2; PLCbeta2

Gene Aliases: AI550384; B230205M18Rik; B230399N12; PLC-beta-2; PLCB2

UniProt ID: (Human) Q00722, (Rat) O89040, (Mouse) A3KGF7

Entrez Gene ID: (Human) 5330, (Rat) 85240, (Mouse) 18796

Molecular Function: phospholipase