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PLCB2 Antibody (PA5-141026) in ICC/IF
Immunocytochemistry analysis of PLCB2 in SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in PLCB2 Polyclonal antibody (Product # PA5-141026) using a dilution of 1:200 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as ... View More
Product Details
PA5-141026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human PLCB2 aa 950-1185.
Conjugate
Form
Liquid
Concentration
1.99 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932478
Target Information
PLCB2 is the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; Phosphoinositide phospholipase C-beta-2; phospholipase C, beta 2; Phospholipase C-beta-2; PLC beta-2; PLC-beta-2; PLC-beta2; PLCbeta-2; PLCbeta2
Gene Aliases: PLC-beta-2; PLCB2
UniProt ID: (Human) Q00722
Entrez Gene ID: (Human) 5330
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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