Invitrogen

PLCB2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (9) PLCB2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PLCB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PLCB2 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PLCB2 Polyclonal Antibody (Product # PA5-78108) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cell membrane and cytosolic localization. Panel e represents SK-O-V3 cells with low expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification.

PLCB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of PLCB2 was performed in paraffin-embedded human colon cancer tissue using PLCB2 Polyclonal Antibody (Product # PA5-78108) at a dilution of 1:500.

PLCB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PLCB2 Polyclonal Antibody (Product # PA5-78108) and a 134 kDa band corresponding to PLCB2 was observed across cell lines. Whole cell extracts (60 µg lysate) of THP-1 (Lane 1), HEL 92.1.7 (Lane 2), NK-92 (Lane 3), Jurkat (Lane 4), HL-60 (Lane 5), U-937 (Lane 6), HeLa (Lane 7), SK-O-V3 (Lane 8) and A549 (Lane 9) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). THP-1, HEL 92.17, NK-92 are high expressing than Jurkat, HL-60, U-937, HeLa, SK-O-V3 and A549 cell lines for PLCB2.

PLCB2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PLCB2 was observed in THP-1, HEL 92.17, NK-92 which are high expressing than Jurkat, HL-60, U-937, HeLa, SK-O-V3 and A549 cell lines using Anti-PLCB2 Polyclonal Antibody (Product # PA5-78108) in Western Blot. {RE}

Product Details

PA5-78108

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of Human PLCB2. The exact sequence is proprietary. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.71 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736444

Product Specific Information

Positive Control: THP-1, HL-60

Predicted Reactivity: Rhesus Monkey (92%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PLCB2 is the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; Phosphoinositide phospholipase C-beta-2; phospholipase C, beta 2; Phospholipase C-beta-2; PLC beta-2; PLC-beta-2; PLC-beta2; PLCbeta-2; PLCbeta2

Gene Aliases: PLC-beta-2; PLCB2

UniProt ID: (Human) Q00722

Entrez Gene ID: (Human) 5330

Molecular Function: phospholipase