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PLCG1 Antibody (PA5-120513) in WB
Western blot analysis of PLCG1 in extracts of various cell lines. Samples were incubated with PLCG1 Polyclonal antibody (Product # PA5-120513) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120513
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PLC gamma 1 (PLCG1) (NP_002651.2).
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914085
Product Specific Information
Positive test controls include: MCF7, Jurkat. The target is usually found in the following locations: Cell projection, lamellipodium, ruffle.
Immunogen sequence: NSYAISFRAE GKIKHCRVQQ EGQTVMLGNS EFDSLVDLIS YYEKHPLYRK MKLRYPINEE ALEKIGTAEP DYGALYEGRN PGFYVEANPM PTFKCAVKAL F
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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