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Invitrogen
PLCG1 Polyclonal Antibody
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FIGURE: 1 / 9
PLCG1 Antibody (PA5-85807) in ICC/IF
Immunofluorescence analysis of PLCG1 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PLCG1 Polyclonal Antibody (Product # PA5-85807) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
Product Details
PA5-85807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLCG1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Amount
62 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: 293T, A431, HeLa, HepG2, Jurkat, Raji, NCI-H929, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; phospholipase C-gamma; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC-II; PLC2I; triphosphoinositide phosphodiesterase; unnamed protein product
Gene Aliases: AI894140; Cded; IDAA; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCG1; PLCgamma1; PPLCA
UniProt ID: (Human) P19174, (Mouse) Q62077, (Rat) P10686
Entrez Gene ID: (Human) 5335, (Mouse) 18803, (Rat) 25738
phosphatidylinositol phospholipase C activity
phospholipase C activity
guanyl-nucleotide exchange factor activity
neurotrophin TRKA receptor binding
calcium ion binding
protein binding
phosphoric diester hydrolase activity
hydrolase activity
protein kinase binding
metal ion binding
calcium-dependent phospholipase C activity
phosphatidylinositol phospholipase C activity
signal transducer activity
insulin receptor binding
receptor tyrosine kinase binding
glutamate receptor binding
phosphoprotein binding
phospholipase
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
signal transduction
epidermal growth factor receptor signaling pathway
phospholipid catabolic process
positive regulation of epithelial cell migration
lipid catabolic process
cell migration
calcium-mediated signaling
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
phosphatidylinositol metabolic process
phosphatidylinositol-mediated signaling
antigen receptor-mediated signaling pathway
T cell receptor signaling pathway
release of sequestered calcium ion into cytosol
positive regulation of release of sequestered calcium ion into cytosol
regulation of sequestering of calcium ion
cellular response to epidermal growth factor stimulus
positive regulation of vascular endothelial cell proliferation
positive regulation of endothelial cell apoptotic process
in utero embryonic development
calcium ion transport
protein secretion
inositol trisphosphate biosynthetic process
regulation of store-operated calcium channel activity
response to gravity
response to organonitrogen compound
response to hydrogen peroxide
response to morphine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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