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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide from near the carboxy terminus of human PLEKHM2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is rat brain tissue lysate.
PA5-20850 can be used with blocking peptide PEP-0964.
PLEKHM2, also known as SKIP, is a member of the M family of Pleckstrin homology domain-containing proteins. While little is known of PLEKHM2, a recent study examining differential gene expression in human hematopoietic stem cells has shown it to be specifically expressed in stem cells, suggesting that PLEKHM2 may play a role in erythroid commitment and development. Other studies have shown that PLEKHM2 is required for interaction with the Salmonella virulence factor SifA for Salmonella pathogenesis, suggesting that PLEKHM2 has cellular roles other than in the developing embryo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
PLEKHM2 mutation leads to abnormal localization of lysosomes, impaired autophagy flux and associates with recessive dilated cardiomyopathy and left ventricular noncompaction.
PA5-20850 was used in western blot to assess the abnormal localization of lysosomes, impaired autophagy flux and association with recessive dilated cardiomyopathy and left ventricular noncompaction by PLEKHM2 mutations
|Muhammad E,Levitas A,Singh SR,Braiman A,Ofir R,Etzion S,Sheffield VC,Etzion Y,Carrier L,Parvari R||Human molecular genetics (24:7227)||2015|
novel RUN and PH domain-containing protein; PH domain-containing family M member 2; pleckstrin homology domain containing, family M (with RUN domain) member 2; Pleckstrin homology domain-containing family M member 2; RP11-169K16.1, SKIP; Salmonella-induced filaments A and kinesin-interacting protein; SifA (Salmonella-induced filaments A) and kinesin-interacting protein; SifA and kinesin-interacting protein
2310034J19Rik; AI854247; KIAA0842; mKIAA0842; PLEKHM2; SKIP