Invitrogen

PLK2 Polyclonal Antibody

Advanced Verification

3 Published Figures

3 References

View all (5) PLK2 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

PLK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

PLK2 Antibody (PA5-14094) in IHC (P)

Immunohistochemistry analysis of PLK2 in formalin-fixed and paraffin-embedded human breast carcinoma tissue. Samples were incubated with PLK2 polyclonal antibody (Product # PA5-14094) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PLK2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

PLK2 Antibody — Figure 3. Hyperactivity induces Plk2 which is required for hyperactivity induction of NF-kappaB. A , Representative immunoblot (inset) and quantification of Plk2 levels in lysates from chronic PTX (PTX, 100 mu m , 24 h) or mock-treated DIV21 hippocampal cultures in the presence (Ctrl or PTX) or absence of p65 (OHT or OHT/PTX). HSC70 was used as the loading control (* p <= 4.54 x 10 -4 , ANOVA; n = 5 independent experiments). B , NF-kappaB luciferase reporter in DIV19 hippocampal neurons. NF-kappaB transcriptional activity is significantly increased by 12 h PTX treatment (50-100 mu m ; * p = 0.003), but this is prevented by expression of the kinase-dead Plk2 K108M ( # p = 0.01; n = 3 independent experiments). C , NF-kappaB limits decreases in spine density by chronic PTX. Hippocampal neurons (DIV21, RelA F/F mice) in the presence or absence of p65 (Cre expression 48 h) were vehicle or PTX treated (100 mu m for 24 h; * p <= 1.62 x 10 -4 , ANOVA; n >= 15 dendritic segments from 4 independent experiments). D , NF-kappaB limits decreases in spine volumes by chronic PTX. Spine head volumes were measured using Imaris software from the same dendritic segments as in A (* p <= 5.42 x 10 -4 , ANOVA). E , Confocal projections of representative dendrite segments for data in C and D . Scale bar, 10 mum. All error bars indicate SEM. {TM}

Immunogen image

Immunogen Image PLK2 Polyclonal Antibody

Product Details

PA5-14094

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 375-406 amino acids from the C-terminal region of human PLK2 (SNK)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

192 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_2167742

Target Information

PLK2 is a serum inducible serine/threonine kinase that is a member of the polo family of serine/threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: h PLK-2; PLK-2; polo-like kinase 1; serine/threonine kinase; serine/threonine-protein kinase SNK; serum-inducible kinase; SNK; unnamed protein product

View more View less

Gene Aliases: hPlk2; hSNK; SNK

View more View less

UniProt ID: (Human) Q9NYY3, (Rat) Q9R012, (Mouse) P53351

View more View less

Entrez Gene ID: (Human) 10769, (Rat) 83722, (Mouse) 20620

View more View less

Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding ATP-dependent protein binding protein serine kinase activity protein kinase binding clathrin heavy chain binding macromolecular complex binding GTPase binding ATPase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PLK2 Polyclonal Antibody

PLK2 Antibody (PA5-14094) in IHC (P)

Product Details

PA5-14094

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!