Invitrogen

PLK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (6) PLK2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PLK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

PLK2 Antibody (PA5-14094) in IHC (P)

Immunohistochemistry analysis of PLK2 in formalin-fixed and paraffin-embedded human breast carcinoma tissue. Samples were incubated with PLK2 polyclonal antibody (Product # PA5-14094) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PLK2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

PLK2 Antibody — Figure 3. Hyperactivity induces Plk2 which is required for hyperactivity induction of NF-kappaB. A , Representative immunoblot (inset) and quantification of Plk2 levels in lysates from chronic PTX (PTX, 100 mu m , 24 h) or mock-treated DIV21 hippocampal cultures in the presence (Ctrl or PTX) or absence of p65 (OHT or OHT/PTX). HSC70 was used as the loading control (* p <= 4.54 x 10 -4 , ANOVA; n = 5 independent experiments). B , NF-kappaB luciferase reporter in DIV19 hippocampal neurons. NF-kappaB transcriptional activity is significantly increased by 12 h PTX treatment (50-100 mu m ; * p = 0.003), but this is prevented by expression of the kinase-dead Plk2 K108M ( # p = 0.01; n = 3 independent experiments). C , NF-kappaB limits decreases in spine density by chronic PTX. Hippocampal neurons (DIV21, RelA F/F mice) in the presence or absence of p65 (Cre expression 48 h) were vehicle or PTX treated (100 mu m for 24 h; * p <= 1.62 x 10 -4 , ANOVA; n >= 15 dendritic segments from 4 independent experiments). D , NF-kappaB limits decreases in spine volumes by chronic PTX. Spine head volumes were measured using Imaris software from the same dendritic segments as in A (* p <= 5.42 x 10 -4 , ANOVA). E , Confocal projections of representative dendrite segments for data in C and D . Scale bar, 10 mum. All error bars indicate SEM. {TM}

Product Details

PA5-14094

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 375-406 amino acids from the C-terminal region of human PLK2 (SNK)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2167742

Target Information

PLK2 is a serum inducible serine/threonine kinase that is a member of the polo family of serine/threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting Adaptive IRE1α Signaling and PLK2 in Multiple Myeloma: Possible Anti-Tumor Mechanisms of KIRA8 and Nilotinib.

International journal of molecular sciences

Yamashita Y,Morita S,Hosoi H,Kobata H,Kishimoto S,Ishibashi T,Mishima H,Kinoshita A,Backes BJ,Yoshiura KI,Papa FR,Sonoki T,Tamura S

Published figure using PLK2 polyclonal antibody (Product # PA5-14094) in Immunohistochemistry

Mon Aug 31 00:00:00 EDT 2020

Targeting Adaptive IRE1α Signaling and PLK2 in Multiple Myeloma: Possible Anti-Tumor Mechanisms of KIRA8 and Nilotinib.

International journal of molecular sciences

Yamashita Y,Morita S,Hosoi H,Kobata H,Kishimoto S,Ishibashi T,Mishima H,Kinoshita A,Backes BJ,Yoshiura KI,Papa FR,Sonoki T,Tamura S

Published figure using PLK2 polyclonal antibody (Product # PA5-14094) in Immunohistochemistry

Mon Aug 31 00:00:00 EDT 2020

Opposing action of nuclear factor κB and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mihalas AB,Araki Y,Huganir RL,Meffert MK

Published figure using PLK2 polyclonal antibody (Product # PA5-14094) in Western Blot

Wed Oct 16 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: h PLK-2; hSNK; PLK-2; polo-like kinase 1; Polo-like kinase 2; Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum-inducible kinase

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Gene Aliases: hPlk2; hSNK; PLK2; SNK

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UniProt ID: (Human) Q9NYY3, (Mouse) P53351, (Rat) Q9R012

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Entrez Gene ID: (Human) 10769, (Mouse) 20620, (Rat) 83722

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Function(s)

protein serine/threonine kinase activity signal transducer activity protein binding ATP binding protein complex binding ATP-dependent protein binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

G1/S transition of mitotic cell cycle protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic spindle organization mitotic cell cycle checkpoint Ras protein signal transduction memory positive regulation of autophagy peptidyl-serine phosphorylation positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process Rap protein signal transduction negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of protein catabolic process regulation of centriole replication regulation of synaptic plasticity long-term synaptic potentiation long term synaptic depression negative regulation of dendritic spine development mitotic cell cycle phosphorylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PLK2 Polyclonal Antibody

PLK2 Antibody (PA5-14094) in IHC (P)

Product Details

PA5-14094

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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