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PLOD1 Antibody (PA5-121155) in WB
Western blot analysis of PLOD1 in extracts of various cell lines. Samples were incubated with PLOD1 Polyclonal antibody (Product # PA5-121155) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-121155
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-295 of human PLOD1 (NP_000293.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
2.60 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2914727
Product Specific Information
Positive test controls include: HepG2, A-431, U-251MG, Mouse kidney, Mouse heart. The target is usually found in the following locations: Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane.
Target Information
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LH1; lysine hydroxylase; lysyl hydroxlase 1; Lysyl hydroxylase 1; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; procollagen-lysine 2-oxoglutarate 5-dioxygenase (lysine hydroxylase Ehlers-Danlos syndrome type VI); procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Aliases: 2410042F05Rik; AI854890; AV007929; EDS6; LH; LH1; LLH; PLOD; PLOD1
UniProt ID: (Human) Q02809, (Mouse) Q9R0E2
Entrez Gene ID: (Human) 5351, (Mouse) 18822, (Rat) 116552
Molecular Function:
protein modifying enzyme
ferrous iron binding
procollagen-lysine 5-dioxygenase activity
L-ascorbic acid binding
peptide binding
protein homodimerization activity
iron ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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