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Invitrogen
PLSCR1 Polyclonal Antibody
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FIGURE: 1 / 3
PLSCR1 Antibody (PA5-77045) in WB
Western blot was performed using Anti-PLSCR1 Polyclonal Antibody (Product # PA5-77045) and a 35 kDa band corresponding to phospholipid scramblase 1 was observed across positive cell lines (THP 1, HaCaT, HCT 116 and Hep G2); and not across negative cell lines (SW480 and SH-SY5Y) tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), SW480 (Lane 2), HaCaT (Lane 3), HCT 116 (Lane 4), Hep G2 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then... View More
Product Details
PA5-77045
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human PLSCR1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The calcium-dependent plasma membrane protein phospholipid scramblase 1 (PLSCR1) contributes to the transbilayer movement of phosphatidylserine and other membrane phospholipids upon influx of calcium into the cytosol. This movement results in plasma membrane phospholipid remodelling and surface exposure of phosphatidylserine in injured or apoptotic cells, which leads to cell death. Interferons and other cytokines induce expression of PLSCR1, implying that PLSCR1 also functions in cytokine signaling pathways. EGF stilmulation results in tyrosine phosphorylation of PLSCR1 on tyrosines 69 and 74, which allows it to interact with Shc, and thereby connecting Src kinase activation to stimulation of the EGF receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; Mg(2+)-dependent nuclease; MmTRA1b; NOR1; Phospholipid scramblase 1; phospholipid scramblase 2; PL scramblase 1; Transplantability-associated protein 1
Gene Aliases: MmTRA1a; MMTRA1B; Nor1; PLSCR1; Tra1; Tra1a; Tra1b; Tras1; Tras2
UniProt ID: (Human) O15162, (Rat) P58195, (Mouse) Q9JJ00
Entrez Gene ID: (Human) 5359, (Rat) 117540, (Mouse) 22038
transcription from RNA polymerase II promoter
phosphatidylserine biosynthetic process
apoptotic process
acute-phase response
positive regulation of gene expression
phospholipid scrambling
platelet activation
regulation of mast cell activation
response to interferon-beta
negative regulation of viral genome replication
positive regulation of innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
regulation of Fc receptor mediated stimulatory signaling pathway
positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
immune response
phospholipid transport
myeloid cell differentiation
negative regulation of protein binding
response to interferon-alpha
positive regulation of apoptotic process
phosphatidylserine exposure on apoptotic cell surface
cellular response to lipopolysaccharide
cellular response to cytokine stimulus
intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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