Invitrogen

PMCA3 ATPase Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (4) PMCA3 ATPase antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PMCA3 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

PMCA3 ATPase Antibody (PA1-916) in IHC (P)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyc... View More

PMCA3 ATPase Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

PMCA3 ATPase Antibody in Immunocytochemistry (ICC/IF)

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Product Details

PA1-916

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunohistochemistry (Frozen) (IHC (F))

1:20

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Pig, Rat

Published species

Human, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: A(5) N S S I E F H P K P Q Q Q R E V P(22)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061588

Product Specific Information

PA1-916 detects calcium pump of the plasma membrane 3 (PMCA3 ATPase) from rat, human and porcine tissues.

PA1-916 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein representing PMCA3a and PMCA3b ATPase from rat brain microsomal fractions.

PA1-916 immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA3 ATPase protein. This sequence is completely conserved between rat and human PMCA3 ATPase.

Target Information

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The ataxia-linked E1081Q mutation affects the sub-plasma membrane Ca²⁺-microdomains by tuning PMCA3 activity.

Cell death & disease

Vallese F,Maso L,Giamogante F,Poggio E,Barazzuol L,Salmaso A,Lopreiato R,Cendron L,Navazio L,Zanni G,Weber Y,Kovacevic-Preradovic T,Keren B,Torraco A,Carrozzo R,Peretto F,Peggion C,Ferro S,Marin O,Zanotti G,Calì T,Brini M,Carafoli E

Published figure using PMCA3 ATPase polyclonal antibody (Product # PA1-916) in Immunocytochemistry

Fri Oct 07 00:00:00 EDT 2022

The ataxia-linked E1081Q mutation affects the sub-plasma membrane Ca²⁺-microdomains by tuning PMCA3 activity.

Cell death & disease

Published figure using PMCA3 ATPase polyclonal antibody (Product # PA1-916) in Immunocytochemistry

Fri Oct 07 00:00:00 EDT 2022

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

PA1-916 was used in Western Blotting to characterize brain region-specific effects of ketamine on PMCA activity.

Thu Oct 01 00:00:00 EDT 2020

Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis.

Proceedings of the National Academy of Sciences of the United States of America

Zanni G,Calì T,Kalscheuer VM,Ottolini D,Barresi S,Lebrun N,Montecchi-Palazzi L,Hu H,Chelly J,Bertini E,Brini M,Carafoli E

PA1-916 was used in immunocytochemistry to study the effects on Ca(2+) homeostasis of a plasma membrane Ca(2+)-ATPase mutation identified in a familiy with X-linked congenital cerebellar ataxia

Tue Sep 04 00:00:00 EDT 2012

Plasma membrane calcium pumps in mouse olfactory sensory neurons.

Chemical senses

Weeraratne SD,Valentine M,Cusick M,Delay R,Van Houten JL

Published figure using PMCA3 ATPase polyclonal antibody (Product # PA1-916) in Immunofluorescence

Sun Oct 01 00:00:00 EDT 2006

The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.

The Journal of biological chemistry

Sepúlveda MR,Berrocal-Carrillo M,Gasset M,Mata AM

PA1-916 was used in western blot to localize the plasma membrane calcium-ATPase isoform 4.

Fri Jan 06 00:00:00 EST 2006

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Investigative ophthalmology & visual science

Tamiya S,Dean WL,Paterson CA,Delamere NA

PA1-916 was used in western blot to investigate the sodium/potassium ATPase protein expression in porcine lens epithelium.

Wed Oct 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: ATPase, Ca++ transporting, plasma membrane 3; cilia and flagella associated protein 39; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 3; plasma membrane calcium pump; Plasma membrane calcium pump isoform 3; Plasma membrane calcium-transporting ATPase 3; PMCA3

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Gene Aliases: ATP2B3; CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1

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UniProt ID: (Human) Q16720, (Rat) Q64568

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Entrez Gene ID: (Human) 492, (Pig) 100520061, (Rat) 29599

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Function(s)

calcium-transporting ATPase activity calmodulin binding ATP binding PDZ domain binding metal ion binding protein binding primary active transporter

Process(es)

transport cellular calcium ion homeostasis ion transmembrane transport calcium ion transmembrane transport regulation of cardiac conduction neural retina development brain development metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PMCA3 ATPase Polyclonal Antibody

PMCA3 ATPase Antibody (PA1-916) in IHC (P)

Product Details

PA1-916

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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