|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near N-terminus of human PML|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lymph node tissue.
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17)(q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor alpha (RARalpha) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARalpha chimeric protein of 106kDa. Myeloid precursor cells expression the PML/RARalpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.