Western blot analysis of Peroxisomal Membrane Protein 70 (PMP70) was performed by loading the following whole cell extracts: 20ug Rat Kidney (Lane 1), 20µg Rat Liver (Lane 2), 20µg Mouse Lung (Lane 3), 10µg A431 cells (Lane 4), and 10µg U2OS cells (Lane 5) per well onto a 4-20% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and the membrane was probed with Pierce Fast Western Kit (Product #35050). The membrane was probed with PMP70 Polyclonal antibody (Product #PA1-650) at a dilution of 1:500 for 30 minutes at room temperature on a rocking platform per Fast Western Kit instructions. The membrane was then washed in kit wash buffer and probed with kit secondary antibody reagent for 10 minutes (diluted at 150µL per 10 mL kit reagent diluents). The membrane was then washed in kit wash buffer and chemiluminescent detection was performed using SuperSignal West Dura (Product #34075).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C N(644) Y E F K K I T E D T V E F G S(659) of rat PMP70.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1.1-650 detects peroxisomal membrane protein 70 from mouse, rat and human tissues.
PA1.1-650 has been successfully used in Western blot, ICC/IF and FACS procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from mouse kidney protein extracts.
The PA1.1-650 immunogen is a synthetic peptide corresponding to residues C N(644) Y E F K K I T E D T V E F G S(659) of rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1.1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.
Peroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum and quote;s disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.