Invitrogen

PMP70 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

19 References

View all (10) PMP70 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PMP70 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PMP70 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PMP70 Rabbit Polyclonal Antibody (Product # PA1-650) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic (peroxisomal pattern) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PMP70 Antibody in Western Blot (WB) — Western blot analysis of Peroxisomal Membrane Protein 70 (PMP70) was performed by loading the following whole cell extracts: 20 µg Rat Kidney (Lane 1), 20 µg Rat Liver (Lane 2), 20 µg Mouse Lung (Lane 3), 10 µg A431 cells (Lane 4), and 10 µg U2OS cells (Lane 5) per well onto a 4-20% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and the membrane was probed with Pierce Fast Western Kit (Product # 35050). The membrane was probed with PMP70 Polyclonal antibody (Product # PA1-650) at a dilution of 1:500 for 30 minutes at room temperature on a rocking platform per Fast Western Kit instructions. The membrane was then washed in kit wash buffer and probed with kit secondary antibody reagent for 10 minutes (diluted at 150 µL per 10 mL kit reagent diluents). The membrane was then washed in kit wash buffer and chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

PMP70 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of PMP70 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PMP70 polyclonal antibody (Product # PA1-650) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

PMP70 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PMP70 siRNA and reduction of specific signal was observed in immunofluorescence application using PMP70 Rabbit Polyclonal Antibody (Product # PA1-650). {KD}

PMP70 Antibody in Immunoprecipitation (IP) — Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Immunoprecipitation

Product Details

PA1-650

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	View 9 publications 9 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1-10 µg/mL	View 7 publications 7 publications
Flow Cytometry (Flow)	0.5 µg/test	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Hamster, Human, Mouse, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659)
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2219912

Product Specific Information

PA1-650 detects peroxisomal membrane protein 70 (PMP70) from mouse tissues.

PA1-650 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from peroxisome-enriched fractions from L cells.

PA1-650 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.

Target Information

Peroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum" disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Symmetrically dispersed spectroscopic single-molecule localization microscopy.

Light, science & applications

Song KH,Zhang Y,Brenner B,Sun C,Zhang HF

PA1-650 was used in SDsSMLM imaging to demonstrate multicolour imaging of fixed cells and three-dimensional single-particle tracking using SDsSMLM.

Fri Nov 05 00:00:00 EDT 2021

The molecular and metabolic program by which white adipocytes adapt to cool physiologic temperatures.

PLoS biology

Mori H,Dugan CE,Nishii A,Benchamana A,Li Z,Cadenhead TS,Das AK,Evans CR,Overmyer KA,Romanelli SM,Peterson SK,Bagchi DP,Corsa CA,Hardij J,Learman BS,El Azzouny M,Coon JJ,Inoki K,MacDougald OA

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Western Blot

Sat May 01 00:00:00 EDT 2021

Autophagy Inhibitors Do Not Restore Peroxisomal Functions in Cells With the Most Common Peroxisome Biogenesis Defect.

Frontiers in cell and developmental biology

Klouwer FCC,Falkenberg KD,Ofman R,Koster J,van Gent D,Ferdinandusse S,Wanders RJA,Waterham HR

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Western Blot

Wed Apr 21 00:00:00 EDT 2021

Coagulopathy in Malnourished Mice Is Sexually Dimorphic and Regulated by Nutrient-Sensing Nuclear Receptors.

Hepatology communications

Preidis GA,Soni KG,Suh JH,Halder T,Kim KH,Choi JM,Li F,Devaraj S,Conner ME,Coarfa C,Jung SY,Moore DD

PA1-650 was used in Western Blotting to show that malnutrition impairs growth and liver synthetic function more severely in male mice than in female mice.

Tue Dec 01 00:00:00 EST 2020

Coagulopathy in Malnourished Mice Is Sexually Dimorphic and Regulated by Nutrient-Sensing Nuclear Receptors.

Hepatology communications

Preidis GA,Soni KG,Suh JH,Halder T,Kim KH,Choi JM,Li F,Devaraj S,Conner ME,Coarfa C,Jung SY,Moore DD

PA1-650 was used in Western Blotting to show that malnutrition impairs growth and liver synthetic function more severely in male mice than in female mice.

Tue Dec 01 00:00:00 EST 2020

Acute inactivation of retromer and ESCPE-1 leads to time-resolved defects in endosomal cargo sorting.

Journal of cell science

Evans AJ,Daly JL,Anuar ANK,Simonetti B,Cullen PJ

PA1-650 was used in Immunocytochemistry-immunoflourescence to show the role for retromer in ESCPE-1-mediated CI-MPR retrograde sorting, and establish a methodology for acute retromer and ESCPE-1 inactivation that will aid the time-resolved dissection of their function role in endosomal cargo sorting.

Mon Aug 03 00:00:00 EDT 2020

Accelerating multicolor spectroscopic single-molecule localization microscopy using deep learning.

Biomedical optics express

Kumar Gaire S,Zhang Y,Li H,Yu R,Zhang HF,Ying L

PA1-650 was used in Immunocytochemistry-immunoflourescence to generate multicolor super-resolution images within a much shorter time, without any changes in the existing sSMLM hardware system.

Fri May 01 00:00:00 EDT 2020

Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy.

Science advances

Mao C,Lee MY,Jhan JR,Halpern AR,Woodworth MA,Glaser AK,Chozinski TJ,Shin L,Pippin JW,Shankland SJ,Liu JTC,Vaughan JC

PA1-650 was used in Immunocytochemistry to report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy.

Fri May 01 00:00:00 EDT 2020

Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.

eLife

Torres SE,Gallagher CM,Plate L,Gupta M,Liem CR,Guo X,Tian R,Stroud RM,Kampmann M,Weissman JS,Walter P

PA1-650 was used in Western Blotting to reveal that Ceapins function by chemical-induced misdirection.

Fri May 31 00:00:00 EDT 2019

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

The Journal of biological chemistry

Young PA,Senkal CE,Suchanek AL,Grevengoed TJ,Lin DD,Zhao L,Crunk AE,Klett EL,Füllekrug J,Obeid LM,Coleman RA

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Western Blot

Fri Oct 26 00:00:00 EDT 2018

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

The Journal of biological chemistry

Young PA,Senkal CE,Suchanek AL,Grevengoed TJ,Lin DD,Zhao L,Crunk AE,Klett EL,Füllekrug J,Obeid LM,Coleman RA

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Western Blot

Fri Oct 26 00:00:00 EDT 2018

UBL3 modification influences protein sorting to small extracellular vesicles.

Nature communications

Ageta H,Ageta-Ishihara N,Hitachi K,Karayel O,Onouchi T,Yamaguchi H,Kahyo T,Hatanaka K,Ikegami K,Yoshioka Y,Nakamura K,Kosaka N,Nakatani M,Uezumi A,Ide T,Tsutsumi Y,Sugimura H,Kinoshita M,Ochiya T,Mann M,Setou M,Tsuchida K

PA1-650 was used in Immunocytochemistry to study the molecular mechanism by which proteins are sorted to small extracellular vesicles.

Wed Sep 26 00:00:00 EDT 2018

LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.

eLife

Lin JP,Mironova YA,Shrager P,Giger RJ

PA1-650 was used in Immunocytochemistry to reveal a novel role for low-density lipoprotein receptor-related protein-1 in peroxisome biogenesis, lipid homeostasis, and oligodendrocyte progenitor cell differentiation during white matter development and repair.

Mon Dec 18 00:00:00 EST 2017

Integrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism.

PLoS pathogens

Sychev ZE,Hu A,DiMaio TA,Gitter A,Camp ND,Noble WS,Wolf-Yadlin A,Lagunoff M

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Immunocytochemistry

Wed Mar 01 00:00:00 EST 2017

Integrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism.

PLoS pathogens

Sychev ZE,Hu A,DiMaio TA,Gitter A,Camp ND,Noble WS,Wolf-Yadlin A,Lagunoff M

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Immunocytochemistry

Wed Mar 01 00:00:00 EST 2017

Peroxisomal protein PEX13 functions in selective autophagy.

EMBO reports

Lee MY,Sumpter R,Zou Z,Sirasanagandla S,Wei Y,Mishra P,Rosewich H,Crane DI,Levine B

Published figure using PMP70 polyclonal antibody (Product # PA1-650) in Immunocytochemistry

Sun Jan 01 00:00:00 EST 2017

Peroxisomal localization of the proopiomelanocortin-derived peptides beta-lipotropin and beta-endorphin.

Endocrinology

Höftberger R,Kunze M,Voigtländer T,Unterberger U,Regelsberger G,Bauer J,Aboul-Enein F,Garzuly F,Forss-Petter S,Bernheimer H,Berger J,Budka H

PA1-650 was used in western blot to study the sub-cellular localisation of beta-lipotropin and beta-endorphin.

Fri Oct 01 00:00:00 EDT 2010

Gene expression analysis in rats treated with experimental acetyl-coenzyme A carboxylase inhibitors suggests interactions with the peroxisome proliferator-activated receptor alpha pathway.

The Journal of pharmacology and experimental therapeutics

Waring JF,Yang Y,Healan-Greenberg CH,Adler AL,Dickinson R,McNally T,Wang X,Weitzberg M,Xu X,Lisowski A,Warder SE,Gu YG,Zinker BA,Blomme EA,Camp HS

PA1-650 was used in immunohistochemistry to investigate the pharmacological functions of small-molecule inhibitors of ACC3 by means of gene expression analysis

Fri Feb 01 00:00:00 EST 2008

Small interfering RNA knockdown of calcium-independent phospholipases A2 beta or gamma inhibits the hormone-induced differentiation of 3T3-L1 preadipocytes.

The Journal of biological chemistry

Su X,Mancuso DJ,Bickel PE,Jenkins CM,Gross RW

PA1-650 was used in immunocytochemistry and western blot to study the effect of calcium-independent phospholipases A2 beta or gamma on 3T3-L1 preadipocyte differentiation

Fri May 21 00:00:00 EDT 2004

Peroxisome proliferator-activated receptor-alpha regulates postischemic liver injury.

American journal of physiology. Gastrointestinal and liver physiology

Okaya T,Lentsch AB

PA1-650 was used in western blot to study the regulation of postischemic liver injury by PPAP alpha

Thu Apr 01 00:00:00 EST 2004

Expression of fatty acid binding proteins inhibits lipid accumulation and alters toxicity in L cell fibroblasts.

American journal of physiology. Cell physiology

Atshaves BP,Storey SM,Petrescu A,Greenberg CC,Lyuksyutova OI,Smith R,Schroeder F

PA1-650 was used in western blot to investigate the role of expression of fatty acid binding proteins on lipid accumulation in L cell fibroblasts

Sun Sep 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: 68 kDa peroxisomal membrane protein; 70 kDa peroxisomal membrane protein; 70-kDa peroxisomal membrane protein; ATP-binding cassette sub-family D member 3; ATP-binding cassette, sub-family D (ALD), member 3; dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)); Peroxisomal membrane protein 1; peroxisomal membrane protein 1 (70kD, Zellweger syndrome); peroxisomal membrane protein, 70 kDa; Peroxisomal membrane protein-1 (70kD); PMP68; PMP70

Gene Aliases: ABC43; ABCD3; AI313901; AU018866; AW146054; CBAS5; PMP68; PMP70; PXMP1; ZWS2

UniProt ID: (Human) P28288, (Mouse) P55096, (Rat) P16970

Entrez Gene ID: (Human) 5825, (Mouse) 19299, (Rat) 25270

Molecular Function: ATP-binding cassette (ABC) transporter