Invitrogen

PMP70 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

2 Published Figures

10 References

View all (9) PMP70 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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PMP70 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of PMP70 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PMP70 Rabbit Polyclonal Antibody (Product # PA1-650) at 5 microgram/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic (peroxisomal pattern) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PMP70 Antibody in Western Blot (WB) — Western blot analysis of Peroxisomal Membrane Protein 70 (PMP70) was performed by loading the following whole cell extracts: 20 µg Rat Kidney (Lane 1), 20 µg Rat Liver (Lane 2), 20 µg Mouse Lung (Lane 3), 10 µg A431 cells (Lane 4), and 10 µg U2OS cells (Lane 5) per well onto a 4-20% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and the membrane was probed with Pierce Fast Western Kit (Product # 35050). The membrane was probed with PMP70 Polyclonal antibody (Product # PA1-650) at a dilution of 1:500 for 30 minutes at room temperature on a rocking platform per Fast Western Kit instructions. The membrane was then washed in kit wash buffer and probed with kit secondary antibody reagent for 10 minutes (diluted at 150 µL per 10 mL kit reagent diluents). The membrane was then washed in kit wash buffer and chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

PMP70 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of PMP70 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PMP70 polyclonal antibody (Product # PA1-650) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

PMP70 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PMP70 siRNA and reduction of specific signal was observed in immunofluorescence application using PMP70 Rabbit Polyclonal Antibody (Product # PA1-650). {KD}

PMP70 Antibody — Published figure using PMP70 polyclonal antibody (Product # PA1-650)

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

Immunocytochemistry (ICC)

1-10 µg/mL

View 4 publications 4 publications

Immunofluorescence (IF)

1-10 µg/mL

View 1 publication 1 publication

Western Blot (WB)

1:500

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2219912

Product Specific Information

PA1-650 detects peroxisomal membrane protein 70 (PMP70) from mouse tissues.

PA1-650 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from peroxisome-enriched fractions from L cells.

PA1-650 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.

Target Information

Peroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum"" disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 68 kDa peroxisomal membrane protein; 70 kDa peroxisomal membrane protein; 70-kDa peroxisomal membrane protein; ATP-binding cassette sub-family D member 3; ATP-binding cassette, sub-family D (ALD), member 3; dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)); Peroxisomal membrane protein 1; peroxisomal membrane protein 1 (70kD, Zellweger syndrome); peroxisomal membrane protein, 70 kDa; Peroxisomal membrane protein-1 (70kD); PMP68; PMP70

Gene Aliases: ABC43; ABCD3; AI313901; AU018866; AW146054; CBAS5; PMP68; PMP70; PXMP1; ZWS2

UniProt ID: (Human) P28288, (Mouse) P55096, (Rat) P16970

Entrez Gene ID: (Human) 5825, (Mouse) 19299, (Rat) 25270

Molecular Function: transporter