Immunofluorescent analysis of PMP70 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PMP70 rabbit polyclonal antibody (Product # PA1-650), at a dilution of 10ug/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Hamster, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||1-10 µg/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-650 detects peroxisomal membrane protein 70 (PMP70) from mouse tissues.
PA1-650 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from peroxisome-enriched fractions from L cells.
PA1-650 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.
Peroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum and quote;s disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Peroxisomal localization of the proopiomelanocortin-derived peptides beta-lipotropin and beta-endorphin.
PA1-650 was used in western blot to study the sub-cellular localisation of beta-lipotropin and beta-endorphin.
|Höftberger R,Kunze M,Voigtländer T,Unterberger U,Regelsberger G,Bauer J,Aboul-Enein F,Garzuly F,Forss-Petter S,Bernheimer H,Berger J,Budka H||Endocrinology (151:4801)||2010|
Small interfering RNA knockdown of calcium-independent phospholipases A2 beta or gamma inhibits the hormone-induced differentiation of 3T3-L1 preadipocytes.
PA1-650 was used in immunocytochemistry and western blot to study the effect of calcium-independent phospholipases A2 beta or gamma on 3T3-L1 preadipocyte differentiation
|Su X,Mancuso DJ,Bickel PE,Jenkins CM,Gross RW||The Journal of biological chemistry (279:21740)||2004|
Peroxisome proliferator-activated receptor-alpha regulates postischemic liver injury.
PA1-650 was used in western blot to study the regulation of postischemic liver injury by PPAP alpha
|Okaya T,Lentsch AB||American journal of physiology. Gastrointestinal and liver physiology (286:G606)||2004|
Expression of fatty acid binding proteins inhibits lipid accumulation and alters toxicity in L cell fibroblasts.
PA1-650 was used in western blot to investigate the role of expression of fatty acid binding proteins on lipid accumulation in L cell fibroblasts
|Atshaves BP,Storey SM,Petrescu A,Greenberg CC,Lyuksyutova OI,Smith R,Schroeder F||American journal of physiology. Cell physiology (283:C688)||2002|
Gene expression analysis in rats treated with experimental acetyl-coenzyme A carboxylase inhibitors suggests interactions with the peroxisome proliferator-activated receptor alpha pathway.
PA1-650 was used in immunohistochemistry to investigate the pharmacological functions of small-molecule inhibitors of ACC3 by means of gene expression analysis
|Waring JF,Yang Y,Healan-Greenberg CH,Adler AL,Dickinson R,McNally T,Wang X,Weitzberg M,Xu X,Lisowski A,Warder SE,Gu YG,Zinker BA,Blomme EA,Camp HS||The Journal of pharmacology and experimental therapeutics (324:507)||2008|