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Invitrogen
PNP Polyclonal Antibody, DyLight™ 488
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FIGURE: 1 / 1
PNP Antibody (PA5-79840) in Flow
Flow Cytometry of PNP in HepG2 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum and then incubated with PNP Polyclonal Antibody, DyLight™ 488 (Product # PA5-79840) at a dilution of 1 μg (per 1x10^6 cells) for 30 min at 20°C.
Product Details
PA5-79840
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189aa AMSDAYDRTMRQRALSTWKQMGEQRELQE).
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control - Flow: HEPG2 cell.
Target Information
The PNP gene encodes for the enzyme purine nucleoside phosphorylase, which plays a vital role in the purine salvage pathway by catalyzing the reversible cleavage of purine nucleosides. This enzyme facilitates the conversion of inosine, guanosine, and deoxyguanosine into free bases and sugars phosphorylated at the C1 position. PNP is located on chromosome 14q13 and is crucial for nucleoside catabolism, impacting energy metabolism and cellular functions across various organisms. PNP deficiency is an autosomal recessive multisystem disorder arising from mutations in the PNP gene, leading to impaired T-cell maturation and function, resulting in recurrent infections, autoimmune manifestations, and neurological abnormalities due to the toxic accumulation of deoxyguanosine triphosphate (dGTP). This deficiency particularly affects immature thymocytes and can lead to lymphoid and neuronal toxicity, underscoring the gene's importance in proper immune system function and cellular health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 156an; HEL-S-156an; Inosine phosphorylase; Inosine-guanosine phosphorylase; NP; PNP; Purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase; unnamed protein product
Gene Aliases: NP; PNP; PRO1837; PUNP
UniProt ID: (Human) P00491
Entrez Gene ID: (Human) 4860
nucleoside binding
purine nucleobase binding
catalytic activity
purine-nucleoside phosphorylase activity
protein binding
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
phosphate ion binding
identical protein binding
guanosine phosphorylase activity
allantoin metabolic process
nucleobase-containing compound metabolic process
inosine catabolic process
deoxyinosine catabolic process
deoxyadenosine catabolic process
purine ribonucleoside salvage
IMP catabolic process
nicotinamide riboside catabolic process
immune response
nucleoside metabolic process
nucleotide biosynthetic process
response to xenobiotic stimulus
positive regulation of interleukin-2 production
urate biosynthetic process
positive regulation of T cell proliferation
purine-containing compound salvage
dAMP catabolic process
positive regulation of alpha-beta T cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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