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Invitrogen
ATGL Polyclonal Antibody
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FIGURE: 1 / 2
ATGL Antibody (PA5-18661) in WB
Western blot was performed using ATGL Polyclonal Antibody (Product # PA5-18661) and a 52 kDa band corresponding to ATGL was observed in adipose tissue only when compared to skeletal muscle. Whole cell extracts (30 µg lysate) of Rat adipose (Lane 1), Rat skeletal muscle (Lane 2) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibod... View More
Product Details
PA5-18661
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (PARPHGPEDKDQ) corresponding to the internal amino acids of PNPLA2 (aa 266-278).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adipose triglyceride lipase; ATGL DESNUTRIN; calcium-independent phospholipase A2; Calcium-independent phospholipase A2-zeta; Desnutrin; DKFZp667M109; IPLA2 zeta; IPLA2-zeta; lipase; missense mutations at exon 2 causes amino acid substitution Leu56Arg; missense mutations at exon 5 causes amino acid substitution Ile193Phe; mutant patatin-like phospholipase domain containing 2; patatin like phospholipase domain containing 2; Patatin-like phospholipase domain-containing protein 2; PEDF R; PEDF-R; pigment epithelium-derived factor; Pigment epithelium-derived factor receptor; plpl; plpl2; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS 2.2; TTS2; TTS2.2
Gene Aliases: 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; PNPLA2; TTS-2.2; TTS2
UniProt ID: (Human) Q96AD5
Entrez Gene ID: (Human) 57104
retinoid metabolic process
lipid metabolic process
acylglycerol metabolic process
diacylglycerol biosynthetic process
phototransduction, visible light
negative regulation of sequestering of triglyceride
positive regulation of triglyceride catabolic process
lipid catabolic process
triglyceride catabolic process
lipid storage
lipid particle organization
cellular triglyceride homeostasis
acylglycerol acyl-chain remodeling
retinol metabolic process
lipid homeostasis
lipid droplet fusion
lipid droplet disassembly
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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