Proteintech

POFUT1 Polyclonal Antibody

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POFUT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

POFUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

POFUT1 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

POFUT1 Antibody in Immunoprecipitation (IP) — IP result of anti-POFUT1 (IP:14929-1-AP, 4ug; Detection:14929-1-AP 1:500) with HepG2 cells lysate 2400ug.

Product Details

14929-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POFUT1 Fusion Protein Ag6710 (1-182 aa encoded by BC000582) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MGAAAWARP LSVSFLLLLL PLPGMPAGSW DPAGYLLYCP CMGRFGNQAD HFLGSLAFAK LLNRTLAVPP WIEYQHHKPP FTNLHVSYQK YFKLEPLQAY HRVISLEDFM EKLAPTHWPP EKRVAYCFEV AAQRSPDKKT CPMKEGNPFG PFWDQFHVSF NKSELFTGIS FSASYREQWS QRR (1-182 aa encoded by BC000582)

Target Information

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.

Cell death & disease

Du Y,Li D,Li N,Su C,Yang C,Lin C,Chen M,Wu R,Li X,Hu G

Mon Sep 24 00:00:00 EDT 2018

POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.

Cell death & disease

Du Y,Li D,Li N,Su C,Yang C,Lin C,Chen M,Wu R,Li X,Hu G

Mon Sep 24 00:00:00 EDT 2018

LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.

Cell death & disease

Liu S,Wang J,Qin HM,Yan XM,Yang XS,Liu C,Yan Q

Thu Aug 28 00:00:00 EDT 2014

Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Annani-Akollor ME,Wang S,Fan J,Liu L,Padhiar AA,Zhang J

Tue Jul 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: GDP-fucose protein O-fucosyltransferase 1; o-fucosyltransferase protein; O-FucT-1; Peptide-O-fucosyltransferase 1; pofut1 {ECO:0000312|RGD:1303001}

Gene Aliases: DDD2; FUT12; KIAA0180; mKIAA0180; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1; POFUT1

UniProt ID: (Human) Q9H488, (Mouse) Q91ZW2, (Rat) Q6EV70

Entrez Gene ID: (Human) 23509, (Mouse) 140484, (Rat) 311551

Molecular Function: protein modifying enzyme