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Invitrogen
POLB Polyclonal Antibody
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Product Details
PA5-79842
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human POLB recombinant protein (Position: L122-E335).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, JURKAT whole cell, SMMC whole cell.
Target Information
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; MGC125976; POL B; POLB; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; unnamed protein product
Gene Aliases: POLB
UniProt ID: (Human) P06746
Entrez Gene ID: (Human) 5423
DNA binding
damaged DNA binding
DNA-directed DNA polymerase activity
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
transferase activity
nucleotidyltransferase activity
lyase activity
enzyme binding
DNA polymerase activity
metal ion binding
5'-deoxyribose-5-phosphate lyase activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
in utero embryonic development
DNA replication
DNA-dependent DNA replication
DNA repair
base-excision repair
base-excision repair, gap-filling
pyrimidine dimer repair
nucleotide-excision repair, DNA gap filling
double-strand break repair via nonhomologous end joining
apoptotic process
inflammatory response
cellular response to DNA damage stimulus
salivary gland morphogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
somatic diversification of immunoglobulins
somatic hypermutation of immunoglobulin genes
response to ethanol
lymph node development
spleen development
homeostasis of number of cells
neuron apoptotic process
response to hyperoxia
immunoglobulin heavy chain V-D-J recombination
DNA biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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