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POLD1 Polyclonal Antibody

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POLD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 15646-1-AP) (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POLD1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

POLD1 Antibody in Immunoprecipitation (IP) — IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug.

Product Details

15646-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POLD1 Fusion Protein Ag8178 (858-1107 aa encoded by BC008800)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: EGAVAHAQD VISDLLCNRI DISQLVITKE LTRAASDYAG KQAHVELAER MRKRDPGSAP SLGDRVPYVI ISAAKGVAAY MKSEDPLFVL EHSLPIDTQY YLEQQLAKPL LRIFEPILGE GRAEAVLLRG DHTRCKTVLT GKVGGLLAFA KRRNCCIGCR TVLSHQGAVC EFCQPRESEL YQKEVSHLNA LEERFSRLWT QCQRCQGSLH EDVICTSRDC PIFYMRKKVR KDLEDQEQLL RRFGPPGPEA W (858-1107 aa encoded by BC008800)

Target Information

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

POLD1 as a Prognostic Biomarker Correlated with Cell Proliferation and Immune Infiltration in Clear Cell Renal Cell Carcinoma.

International journal of molecular sciences

Tian J,Cheng C,Gao J,Fu G,Xu Z,Chen X,Wu Y,Jin B

Thu Apr 06 00:00:00 EDT 2023

POLD1 as a Prognostic Biomarker Correlated with Cell Proliferation and Immune Infiltration in Clear Cell Renal Cell Carcinoma.

International journal of molecular sciences

Tian J,Cheng C,Gao J,Fu G,Xu Z,Chen X,Wu Y,Jin B

Thu Apr 06 00:00:00 EDT 2023

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Kim H,Villareal LB,Liu Z,Haneef M,Falcon DM,Martin DR,Lee HJ,Dame MK,Attili D,Chen Y,Varani J,Spence JR,Kovbasnjuk O,Colacino JA,Lyssiotis CA,Lin HC,Shah YM,Xue X

Sat Apr 01 00:00:00 EDT 2023

Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma.

Journal of translational medicine

Jeon H,Byun J,Kang H,Kim K,Lee E,Kim JH,Hong CK,Song SW,Kim YH,Chong S,Kim JH,Nam SJ,Park JE,Lee S

Thu Feb 02 00:00:00 EST 2023

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Cell

Mo X,Niu Q,Ivanov AA,Tsang YH,Tang C,Shu C,Li Q,Qian K,Wahafu A,Doyle SP,Cicka D,Yang X,Fan D,Reyna MA,Cooper LAD,Moreno CS,Zhou W,Owonikoko TK,Lonial S,Khuri FR,Du Y,Ramalingam SS,Mills GB,Fu H

Thu May 26 00:00:00 EDT 2022

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Oncology letters

Yang X,Li Y,Liu G,Zha W,Liu Y

Sun May 01 00:00:00 EDT 2022

ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.

Nature cancer

Zhang T,Sun L,Hao Y,Suo C,Shen S,Wei H,Ma W,Zhang P,Wang T,Gu X,Li ST,Chen Z,Yan R,Zhang Y,Cai Y,Zhou R,Jia W,Huang F,Gao P,Zhang H

Sat Jan 01 00:00:00 EST 2022

Hepatitis B virus cccDNA is formed through distinct repair processes of each strand.

Nature communications

Wei L,Ploss A

Thu Mar 11 00:00:00 EST 2021

Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation.

Nature microbiology

Wei L,Ploss A

Fri May 01 00:00:00 EDT 2020

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Haematologica

Maio N,Kim KS,Holmes-Hampton G,Singh A,Rouault TA

Sun Sep 01 00:00:00 EDT 2019

Elevated expression of POLD1 is associated with poor prognosis in breast cancer.

Oncology letters

Qin Q,Tan Q,Li J,Yang W,Lian B,Mo Q,Wei C

Thu Nov 01 00:00:00 EDT 2018

Elevated expression of POLD1 is associated with poor prognosis in breast cancer.

Oncology letters

Qin Q,Tan Q,Li J,Yang W,Lian B,Mo Q,Wei C

Thu Nov 01 00:00:00 EDT 2018

ISCA1 mutation in a patient with infantile-onset leukodystrophy causes defects in mitochondrial [4Fe-4S] proteins.

Human molecular genetics

Torraco A,Stehling O,Stümpfig C,Rösser R,De Rasmo D,Fiermonte G,Verrigni D,Rizza T,Vozza A,Di Nottia M,Diodato D,Martinelli D,Piemonte F,Dionisi-Vici C,Bertini E,Lill R,Carrozzo R

Mon Oct 15 00:00:00 EDT 2018

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Human molecular genetics

Kim KS,Maio N,Singh A,Rouault TA

Thu Mar 01 00:00:00 EST 2018

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Human molecular genetics

Kim KS,Maio N,Singh A,Rouault TA

Thu Mar 01 00:00:00 EST 2018

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.

The Journal of biological chemistry

Upadhyay AS,Stehling O,Panayiotou C,Rösser R,Lill R,Överby AK

Fri Aug 18 00:00:00 EDT 2017

p53 inhibits the expression of p125 and the methylation of POLD1 gene promoter by downregulating the Sp1-induced DNMT1 activities in breast cancer.

OncoTargets and therapy

Zhang L,Yang W,Zhu X,Wei C

Tue Mar 29 00:00:00 EDT 2016

MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.

Science (New York, N.Y.)

Stehling O,Vashisht AA,Mascarenhas J,Jonsson ZO,Sharma T,Netz DJ,Pierik AJ,Wohlschlegel JA,Lill R

Fri Jul 13 00:00:00 EDT 2012

MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.

Science (New York, N.Y.)

Stehling O,Vashisht AA,Mascarenhas J,Jonsson ZO,Sharma T,Netz DJ,Pierik AJ,Wohlschlegel JA,Lill R

Fri Jul 13 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 3'-5' exodeoxyribonuclease; CDC2 homolog; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; POL D; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; Polymerase D

Gene Aliases: CDC2; CRCS10; MDPL; POLD; POLD1

UniProt ID: (Human) P28340

Entrez Gene ID: (Human) 5424

Molecular Function: DNA metabolism protein

Function(s)

telomere maintenance via recombination telomere maintenance DNA synthesis involved in DNA repair DNA replication DNA strand elongation involved in DNA replication DNA repair transcription-coupled nucleotide-excision repair base-excision repair, gap-filling nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair response to UV translesion synthesis nucleotide-excision repair, DNA incision cellular response to UV DNA damage response, detection of DNA damage DNA replication proofreading fatty acid homeostasis

Process(es)

nucleotide binding DNA binding chromatin binding damaged DNA binding DNA-directed DNA polymerase activity protein binding 3'-5'-exodeoxyribonuclease activity enzyme binding metal ion binding 4 iron, 4 sulfur cluster binding

POLD1 Polyclonal Antibody

Product Details

15646-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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