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Invitrogen
POLD3 Polyclonal Antibody
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FIGURE: 1 / 2
POLD3 Antibody (PA5-36951) in WB
Western blot analysis of POLD3 in Lane 1: HuT78 whole cell lysate (40 µg), Lane 2: Myla2059 whole cell lysate (40 µg), Lane 3: SGC7901 whole cell lysate (40 µg), Lane 4: the Testis tissue lysate of mouse (40 µg), Lane 5: PC12 whole cell lysate (40 µg). Samples were incubated with POLD3 polyclonal antibody (Product # PA5-36951) at a dilution of 1:500.
Product Details
PA5-36951
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the 421-466 of human DNA pol delta 3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 51 kDa.
Purity is >95% by SDS-PAGE.
Target Information
DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, exonucleases are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases, namely DNA pol alpha, beta, delta and epsilon. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits which interact with the sliding DNA clamp protein PCNA. DNA pol delta 3, also known as POLD3 or p66, is a 466 amino acid nuclear protein that is required for the proper function of DNA pol delta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA pol delta3; DNA polymerase delta subunit 3; DNA polymerase delta subunit C; DNA polymerase delta subunit p66; DNA polymerase delta subunit p68; DNA-directed DNA polymerase delta 3; DNApol delta3; GC12; KIAA0039; Pol delta C subunit (p66); polymerase (DNA directed), delta 3; polymerase (DNA) delta 3, accessory subunit; polymerase (DNA-directed), delta 3, accessory subunit; protein phosphatase 1, regulatory subunit 128; unnamed protein product
Gene Aliases: 2410142G14Rik; C85233; IMD122; KIAA0039; P66; P68; POLD3; PPP1R128
UniProt ID: (Human) Q15054, (Mouse) Q9EQ28
Entrez Gene ID: (Human) 10714, (Rat) 293144, (Mouse) 67967
DNA synthesis involved in DNA repair
DNA replication
DNA-dependent DNA replication
DNA strand elongation involved in DNA replication
DNA repair
nucleotide-excision repair, DNA gap filling
mismatch repair
cellular response to DNA damage stimulus
error-prone translesion synthesis
DNA biosynthetic process
DNA synthesis involved in UV-damage excision repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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