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Invitrogen
POLE Polyclonal Antibody
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FIGURE: 1 / 3
POLE Antibody (PA5-78113) in ICC/IF
POLE Polyclonal Antibody detects POLE protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLE protein stained by POLE Polyclonal Antibody (Product # PA5-78113) diluted at 1:2,500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Product Details
PA5-78113
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human POLE. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.45 mg/mL
Amount
145 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: K562
Predicted Reactivity: Mouse (90%), Rat (90%), Bovine (94%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
POLE (Polepsi) is present in proliferating cells and is implicated in repair of UV-damaged DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; DNA polymerase epsilon catalytic subunit A; DNA polymerase epsilon catalytic subunit protein; DNA polymerase II subunit A; DNA-directed DNA polymerase epsilon; pol-epsilon; polymerase (DNA directed), epsilon, catalytic subunit; polymerase (DNA) epsilon, catalytic subunit; unnamed protein product
Gene Aliases: CRCS12; FILS; IMAGEI; POLE; POLE1
UniProt ID: (Human) Q07864, (Mouse) Q9WVF7
Entrez Gene ID: (Human) 5426, (Mouse) 18973
nucleotide binding
nucleic acid binding
DNA binding
chromatin binding
DNA-directed DNA polymerase activity
protein binding
zinc ion binding
single-stranded DNA 3'-5' exodeoxyribonuclease activity
transferase activity
nucleotidyltransferase activity
hydrolase activity
DNA polymerase activity
metal ion binding
iron-sulfur cluster binding
4 iron, 4 sulfur cluster binding
3'-5' exonuclease activity
DNA metabolism protein
G1/S transition of mitotic cell cycle
mitotic cell cycle
DNA synthesis involved in DNA repair
DNA replication
DNA-dependent DNA replication
leading strand elongation
DNA repair
base-excision repair, gap-filling
nucleotide-excision repair, DNA gap filling
cellular response to DNA damage stimulus
DNA replication proofreading
embryonic organ development
DNA biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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