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Invitrogen
POLE3 Polyclonal Antibody
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FIGURE: 1 / 2
POLE3 Antibody (PA5-92999) in WB
Western blot analysis of POLE3 in extracts of various cell lines. Samples were incubated with POLE3 Polyclonal antibody (Product # PA5-92999) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-92999
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_0591393)
Conjugate
Form
Liquid
Concentration
0.44 mg/mL
Amount
44 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS CANNFAMKGK RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK EASEQKKKDK DKKTDSEEQD KSRDEDNDED EERLEEEEQN EEEEVDN; Positive Samples: HepG2, Mouse testis, Mouse bone marrow, Mouse spleen; Cellular Location: Nucleus
Target Information
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: arsenic transactivated protein; Arsenic-transactivated protein; AsTP; CHRAC 17; CHRAC-17; Chromatin accessibility complex 17 kDa protein; chromatin accessibility complex subunit 2; DNA polymerase epsilon p17 subunit; DNA polymerase epsilon subunit 3; DNA polymerase epsilon subunit p17; DNA polymerase epsilon, subunit 3; DNA polymerase II subunit 3; histone fold protein CHRAC17; huCHRAC17; NF-YB-like protein; polymerase (DNA directed), epsilon 3 (p17 subunit); polymerase (DNA directed), epsilon 3, accessory subunit; polymerase (DNA) epsilon 3, accessory subunit; unnamed protein product; YB-like protein 1; YBL1
Gene Aliases: 1810034K18Rik; CHARAC17; CHRAC17; CHRAC2; p17; POLE3; YBL1
UniProt ID: (Human) Q9NRF9, (Mouse) Q9JKP7
Entrez Gene ID: (Human) 54107, (Mouse) 59001
negative regulation of transcription from RNA polymerase II promoter
DNA replication
DNA-dependent DNA replication
leading strand elongation
regulation of DNA replication
nucleosome assembly
chromatin remodeling
cellular response to DNA damage stimulus
heterochromatin assembly
DNA biosynthetic process
histone H3 acetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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