Invitrogen

POLG2 Polyclonal Antibody

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POLG2 Antibody in Western Blot (WB) — Western blot analysis of POLG2 in 293T, MCF7. Samples were incubated in polyclonal POLG2 antibody (Product # PA5-110196) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

POLG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of POLG2 in paraffin-embedded mouse testis tissue. Samples were incubated in polyclonal POLG2 antibody (Product # PA5-110196) using a dilution of 1:100.

POLG2 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry analysis of POLG2 in MCF7 cells. Samples were incubated in polyclonal POLG2 antibody (Product # PA5-110196) using a dilution of 1:50, followed by DAPI.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:10-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).

Conjugate

Unconjugated

Form

Liquid

Concentration

0.59 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2855607

Product Specific Information

Sequence of this protein is as follows: HPVFDTKQIR NGVKSIGEKT EASLVWFTPP RTSNQWLDFW LRHRLQWWRK FAMSPSNFSS SDCQDEEGRK GNKLYYNFPW GKELIETLWN LGDHELLHMY PGNVSKLHGR DGRKNVVPCV LSVNGDLDRG MLAYLYDSFQ LTENSFTRKK NLHRKVLKLH PCLAPIKVAL DVGRGPTLEL RQVCQGLFNE LLENGISVWP GYLETMQSSL EQLYSKYDEM SILFTVLVTE TTLENGLIHL RSRDTTMKEM MHISKLKDFL IKYISSAKNV

Target Information

This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA polymerase gamma; DNA polymerase gamma accessory 55 kDa subunit; DNA polymerase gamma subunit 1 (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha); DNA polymerase subunit gamma-1; DNA polymerase subunit gamma-2, mitochondrial; DNA-directed DNA polymerase gamma; Mitochondrial DNA polymerase accessory subunit; Mitochondrial DNA polymerase catalytic subunit; mitochondrial DNA polymerase gamma; mitochondrial DNA polymerase gamma catalytic subunit; mitochondrial DNA polymerase subunit gamma-2; mitochondrial DNA polymerase, accessory subunit; mitochondrial DNA polymerase-gamma; mitochondrial polymerase gamma; mitochondrial polymerase gamma 2; MtPolB; p55; Pol gamma; PolG-alpha; PolG-beta; polymerase (DNA directed), gamma; polymerase (DNA directed), gamma 2, accessory subunit; polymerase (DNA) gamma 2, accessory subunit; polymerase (DNA) gamma, catalytic subunit; polymerase, gamma; polymerase, gamma 2; truncated mitochondrial DNA polymerase gamma catalytic subunit

Gene Aliases: AA409516; HP55; MDP1; Mip1; MIRAS; MTDPS4A; MTDPS4B; MTPOLB; PEO; PEOA4; POLB; POLG; POLG-BETA; POLG1; POLG2; POLGA; POLGB; SANDO; SCAE

UniProt ID: (Human) P54098, (Human) Q9UHN1, (Rat) Q9QYV8, (Mouse) Q9QZM2

Entrez Gene ID: (Human) 5428, (Human) 11232, (Mouse) 18975, (Rat) 85472, (Rat) 303612, (Mouse) 50776

Molecular Function: DNA binding protein DNA-directed DNA polymerase exodeoxyribonuclease nuclease nucleic acid binding nucleotidyltransferase transferase aminoacyl-tRNA synthetase ligase