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POLG2 Antibody (PA5-110196) in WB
Western blot analysis of POLG2 in extracts of various cell lines. Samples were incubated with POLG2 Polyclonal antibody (Product # PA5-110196) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-110196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.01 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2855607
Product Specific Information
Sequence of this protein is as follows: HPVFDTKQIR NGVKSIGEKT EASLVWFTPP RTSNQWLDFW LRHRLQWWRK FAMSPSNFSS SDCQDEEGRK GNKLYYNFPW GKELIETLWN LGDHELLHMY PGNVSKLHGR DGRKNVVPCV LSVNGDLDRG MLAYLYDSFQ LTENSFTRKK NLHRKVLKLH PCLAPIKVAL DVGRGPTLEL RQVCQGLFNE LLENGISVWP GYLETMQSSL EQLYSKYDEM SILFTVLVTE TTLENGLIHL RSRDTTMKEM MHISKLKDFL IKYISSAKNV
Target Information
This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase gamma accessory 55 kDa subunit; DNA polymerase subunit gamma-2, mitochondrial; Mitochondrial DNA polymerase accessory subunit; mitochondrial DNA polymerase subunit gamma-2; mitochondrial DNA polymerase, accessory subunit; mitochondrial polymerase gamma 2; MtPolB; p55; PolG-beta; polymerase (DNA directed), gamma 2, accessory subunit; polymerase (DNA) gamma 2, accessory subunit; polymerase, gamma 2
Gene Aliases: HP55; MTPOLB; PEOA4; POLB; POLG-BETA; POLG2; POLGB
UniProt ID: (Human) Q9UHN1, (Mouse) Q9QZM2
Entrez Gene ID: (Human) 11232, (Rat) 303612, (Mouse) 50776
Molecular Function:
aminoacyl-tRNA synthetase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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