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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 12 and 292 of Human POLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-29063 targets POLH in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29063 immunogen is recombinant fragment corresponding to a region within amino acids 12 and 292 of Human POLH.
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Correlation of phenotype/genotype in a cohort of 23 xeroderma pigmentosum-variant patients reveals 12 new disease-causing POLH mutations.
PA5-29063 was used in western blot to perform phenotypic and genotypic comparisons of patients with xeroderma pigmentosum variant disease and study 12 novel POLH disease-causing mutations
|Opletalova K,Bourillon A,Yang W,Pouvelle C,Armier J,Despras E,Ludovic M,Mateus C,Robert C,Kannouche P,Soufir N,Sarasin A||Human mutation (35:117)||2014|
polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30; RAD30 homolog A; RAD30A; xeroderma pigmentosum variant type protein; XP-V; XPV
POLH; RAD30; RAD30A; XP-V; XPV