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Invitrogen
POLH Polyclonal Antibody
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FIGURE: 1 / 4
POLH Antibody (PA5-76055) in ICC/IF
Immunofluorescence analysis of POLH was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with POLH Rabbit Polyclonal Antibody (Product # PA5-76055) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
PA5-76055
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human POLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The XPA gene encodes a zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (POL H) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA polymerase eta; DNA polymerase eta transcript; DNA-directed DNA polymerase eta; FLJ16395; FLJ21978; polymerase (DNA directed), eta; polymerase (DNA directed), eta (RAD 30 related); polymerase (DNA) eta; RAD30 homolog A; Saccharomyces cerevisiae RAD30-encoded Pol eta homolog; unnamed protein product; Xeroderma pigmentosum variant type protein; Xeroderma pigmentosum variant type protein homolog; XP V
Gene Aliases: POLH; RAD30; RAD30A; XP-V; XPV
UniProt ID: (Human) Q9Y253, (Mouse) Q9JJN0
Entrez Gene ID: (Human) 5429, (Rat) 316235, (Mouse) 80905
DNA synthesis involved in DNA repair
DNA replication
DNA repair
regulation of DNA repair
pyrimidine dimer repair
postreplication repair
cellular response to DNA damage stimulus
response to radiation
response to UV-C
error-prone translesion synthesis
error-free translesion synthesis
cellular response to UV-C
DNA biosynthetic process
biological_process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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