Search
Invitrogen
POLQ Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
POLQ Antibody (PA5-69577) in WB
Nature communications 2019 -
Fig. 1 Poltheta promotes efficient MMEJ of long ssDNA. a Schematic of Poltheta. b Schematic of MMEJ of DNA with long overhangs. c Schematic of Poltheta-pol ssDNAx via snap-back replication. d Schematic of Poltheta MMEJ of long ssDNA. e SDS gel (left) and Western blot (right) of purified human Poltheta. f Denaturing gel showing primer extension by Poltheta and Poltheta-pol. g TLC plate showing ... View More
Product Details
PA5-69577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the C terminal region of human POLQ
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 86%; Rat: 100%
Target Information
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA polymerase eta; DNA polymerase theta; DNA-directed; EC 2.7.7.7; epididymis secretory sperm binding protein; polymerase (DNA directed), theta; polymerase (DNA) theta
Gene Aliases: POLH; POLQ; PRO0327
UniProt ID: (Human) O75417
Entrez Gene ID: (Human) 10721
nucleotide binding
magnesium ion binding
nucleic acid binding
DNA binding
DNA helicase activity
chromatin binding
damaged DNA binding
catalytic activity
DNA-directed DNA polymerase activity
RNA-directed DNA polymerase activity
helicase activity
protein binding
ATP binding
transferase activity
nucleotidyltransferase activity
hydrolase activity
ATPase activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
DNA polymerase activity
identical protein binding
5'-deoxyribose-5-phosphate lyase activity
DNA synthesis involved in DNA repair
DNA replication
DNA-dependent DNA replication
RNA-dependent DNA biosynthetic process
DNA repair
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
somatic hypermutation of immunoglobulin genes
replication fork processing
error-prone translesion synthesis
protein homooligomerization
double-strand break repair via alternative nonhomologous end joining
negative regulation of double-strand break repair via homologous recombination
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support