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POLR2F Antibody (PA5-103803) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded POLR2F in rat lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with POLR2F polyclonal antibody (Product # PA5-103803) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-103803
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POLR2F(Accession P61218), corresponding to amino acid residues M1-L39.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853136
Product Specific Information
Antibody detects endogenous levels of total RPAB2.
Target Information
DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC2; hsRPB6; polymerase (RNA) II (DNA directed) polypeptide F; polymerase (RNA) II subunit F; polymerase II; RNA Pol II; RNA Polymerase II subunit 14.4 kD; RNA polymerases I, II, and III subunit ABC2; RPABC14.4; RPB14.4; RPB6 homolog; RPC15
Gene Aliases: 1810060D16Rik; HRBP14.4; POLR2F; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15
UniProt ID: (Human) P61218, (Mouse) P61219, (Rat) O88828
Entrez Gene ID: (Human) 5435, (Mouse) 69833, (Rat) 83503
mRNA splicing, via spliceosome
transcription-coupled nucleotide-excision repair
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
transcription from RNA polymerase III promoter
fibroblast growth factor receptor signaling pathway
gene expression
gene silencing by RNA
positive regulation of type I interferon production
somatic stem cell population maintenance
snRNA transcription from RNA polymerase II promoter
positive regulation of gene expression, epigenetic
positive regulation of viral transcription
transcription, DNA-templated
transcription from RNA polymerase I promoter
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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