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          • Primary Antibodies ›
          • POLR2F Antibodies

          Invitrogen

          POLR2F Polyclonal Antibody

          View all (8) POLR2F antibodies

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          Cite POLR2F Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          POLR2F Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          POLR2F Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          POLR2F Antibody (PA5-51462) in ICC/IF

          Immunofluorecent analysis of POLR2F in human cell line U-251 MG using POLR2F Polyclonal Antibody (Product # PA5-51462). Staining shows localization to nucleus and nucleoli fibrillar center. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          POLR2F Antibody in Immunocytochemistry (ICC/IF)
          POLR2F Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          POLR2F Antibody in Western Blot (WB)
          POLR2F Polyclonal Antibody

          Product Details

          PA5-51462

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human POLR2F. Recombinant protein control fragment (Product #RP-91353).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Amount

          10 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2645759

          Product Specific Information

          Immunogen sequence: GDDFDDVEED EGLDDLENAE EEGQENVEIL PSGERPQANQ KRITTPYMTK YERARVLGTR ALQIAMCAPV MVELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELII

          Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

          Target Information

          DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC2; hsRPB6; POLR2F; polymerase (RNA) II (DNA directed) polypeptide F; polymerase (RNA) II subunit F; RNA Polymerase II subunit 14.4 kD; RNA polymerase II subunit F; RNA polymerases I, II, and III subunit ABC2; RPABC14.4; RPB14.4; RPB6 homolog; RPC15; unnamed protein product

          View more View less

          Gene Aliases: HRBP14.4; POLR2F; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15

          View more View less

          UniProt ID: (Human) P61218

          View more View less

          Entrez Gene ID: (Human) 5435

          View more View less

          Function(s)
          DNA binding DNA-directed RNA polymerase activity
          Process(es)
          transcription, DNA-templated transcription from RNA polymerase I promoter transcription from RNA polymerase II promoter tRNA transcription from RNA polymerase III promoter
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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