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POLR2F Antibody (PA5-51462) in WB
Western blot analysis of POLR2F in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR2F Polyclonal Antibody (Product # PA5-51462).
Product Details
PA5-51462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human POLR2F. Recombinant protein control fragment (Product #RP-91353).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2645759
Product Specific Information
Immunogen sequence: GDDFDDVEED EGLDDLENAE EEGQENVEIL PSGERPQANQ KRITTPYMTK YERARVLGTR ALQIAMCAPV MVELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELII
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC2; hsRPB6; polymerase (RNA) II (DNA directed) polypeptide F; polymerase (RNA) II subunit F; RNA Polymerase II subunit 14.4 kD; RNA polymerases I, II, and III subunit ABC2; RPABC14.4; RPB14.4; RPB6 homolog; RPC15
Gene Aliases: HRBP14.4; POLR2F; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15
UniProt ID: (Human) P61218
Entrez Gene ID: (Human) 5435
mRNA splicing, via spliceosome
transcription-coupled nucleotide-excision repair
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
transcription from RNA polymerase III promoter
fibroblast growth factor receptor signaling pathway
gene expression
gene silencing by RNA
positive regulation of type I interferon production
somatic stem cell population maintenance
snRNA transcription from RNA polymerase II promoter
positive regulation of gene expression, epigenetic
positive regulation of viral transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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