Proteintech

POLR2H Polyclonal Antibody

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POLR2H Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

POLR2H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 40x lens).

POLR2H Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

POLR2H Antibody in Immunoprecipitation (IP) — IP result of anti-POLR2H (IP:15086-1-AP, 4ug; Detection:15086-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

Product Details

15086-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLR2H Fusion Protein Ag7166 (1-150 aa encoded by BC000739) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.41 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAGILFEDI FDVKDIDPEG KKFDRVSRLH CESESFKMDL ILDVNIQIYP VDLGDKFRLV IASTLYEDGT LDDGEYNPTD DRPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F (1-150 aa encoded by BC000739)

Target Information

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5. 8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of the key genes and pathways in prostate cancer.

Oncology letters

Fan S,Liang Z,Gao Z,Pan Z,Han S,Liu X,Zhao C,Yang W,Pan Z,Feng W

Thu Nov 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: ABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; hsRPB8; polymerase (RNA) II (DNA directed) polypeptide H; polymerase (RNA) II subunit H; RNA polymerases I, II, and III subunit ABC3; RPB17; RPB8 homolog

Gene Aliases: POLR2H; RGD1561203; RPABC3; RPB17; RPB8

UniProt ID: (Human) P52434, (Mouse) Q923G2

Entrez Gene ID: (Human) 5437, (Mouse) 245841, (Rat) 498109

Molecular Function: DNA-directed RNA polymerase