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POLR3K Antibody (PA5-92997) in WB
Western blot analysis of POLR3K in extracts of various cell lines. Samples were incubated with POLR3K Polyclonal antibody (Product # PA5-92997) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92997
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_0573943)
Conjugate
Form
liquid
Concentration
0.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806579
Product Specific Information
Immunogen sequence: MLLFCPGCGN GLIVEEGQRC HRFACNTCPY VHNITRKVTN RKYPKLKEVD DVLGGAAAWE NVDSTAESCP KCEHPRAYFM QLQTRSADEP MTTFYKCCNA QCGHRWRD; Positive Samples: MCF7, SW620; Cellular Location: Nucleus, nucleolus
Target Information
Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. POLR3K, also known as DNA-directed RNA polymerase III subunit RPC11, is a 108 amino acid nuclear protein that is one of the seventeen subunits that comprise Pol III. Specifically, POLR3K is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA. The gene encoding POLR3K maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs and is associated with a variety of genetic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase III subunit K; DNA-directed RNA polymerase III subunit RPC10; DNA-directed RNA polymerases III 12.5 kDa polypeptide; hRPC11; HsC11p; polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa; polymerase (RNA) III subunit K; RNA polymerase III 12.5 kDa subunit; RNA polymerase III subunit C10; RNA polymerase III subunit C11; RNA polymerase III subunit CII; RPC12.5
Gene Aliases: C11; C11-RNP3; My010; POLR3K; RPC10; RPC11; RPC12.5
UniProt ID: (Human) Q9Y2Y1
Entrez Gene ID: (Human) 51728
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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