Proteintech

POM121 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

POM121 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 15645-1-AP (POM121 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

POM121 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 15645-1-AP ( POM121 Antibody) at dilution of 1:50 (under 40x lens).

POM121 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

POM121 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

15645-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:10-1:100	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POM121 Fusion Protein Ag8177 (635-984 aa encoded by BC008794) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

Immunogen sequence: SQSLHTAVP TATSSSAADF SGFGSTLATS APATSSQPTL TFSNTSTPTF NIPFGSSAKS PLPSYPGANP QPAFGAAEGQ PPGAAKPALA PSFGSSFTFG NSAAPAAAPT PAPPSMIKVV PAYVPTPIHP IFGGATHSAF GLKATASAFG APASSQPAFG GSTAVFFGAA TSSGFGATTQ TASSGSSSSV FGSTTPSPFT FGGSAAPAGS GSFGINVATP GSSTTTGAFS FGAGQSGSTA TSTPFAGGLG QNALGTTGQS TPFAFNVSST TESKPVFGGT ATPTFGLNTP APGVGTSGSS LSFGASSAPA QGFVGVAPFG SAALSFSIGA GSKTPGARQR LQARRQHTRK K (635-984 aa encoded by BC008794)

Target Information

POM121 (POM121 membrane glycoprotein), also known as POM121A (nuclear envelope pore membrane protein POM 121A), NUP121 (nucleoporin Nup121) or pore membrane protein of 121 kDa, is a 1,249 amino acid single-pass membrane protein that exists as three alternatively spiced isoforms and belongs to the POM121 family. Encoded by a gene that maps to human chromosome 7q11.23, POM121 is highly conserved and localizes to nucleus membrane and endoplasmic reticulum membrane. An essential component of the nuclear pore complex (NPC), POM121 is mandatory for nuclear envelope formation and may play a role in biogenesis of the NPC. POM121 associates with the central spoke ring complex and is involved in anchoring components of the pore complex to the pore membrane by way of its F-X-F-G repeat-containing domain. POM121 overexpression induces the formation of cytoplasmic annulate lamellae (AL).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

eLife

Kane M,Rebensburg SV,Takata MA,Zang TM,Yamashita M,Kvaratskhelia M,Bieniasz PD

Tue Aug 07 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Nuclear envelope pore membrane protein POM 121; Nuclear envelope pore membrane protein POM 121A; nuclear pore membrane glycoprotein 121 kD; nuclear pore membrane protein 121; nuclear pore membrane protein 121 kDa; nuclear pore membrane protein 121-2; Nucleoporin Nup121; P145; POM121 membrane glycoprotein; POM121 membrane glycoprotein C; POM121B; Pore membrane protein of 121 kDa; pore membrane protein of 121 kDa C

Gene Aliases: 2610027A18Rik; C80273; KIAA0618; mKIAA0618; NUP121; P145; POM121; POM121-2; POM121A

UniProt ID: (Human) Q96HA1, (Rat) P52591, (Mouse) Q8K3Z9

Entrez Gene ID: (Human) 9883, (Human) 100101267, (Rat) 113975, (Mouse) 107939

Molecular Function: transporter