Proteintech

POT1 Polyclonal Antibody

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Antibody Testing Data (7)

POT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

POT1 Antibody (10581-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 40x lens).

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Product Details

10581-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POT1 Fusion Protein Ag0875 (336-634 aa encoded by BC002923)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ILTDHQYLE RTPLCAILKQ KAPQQYRIRA KLRSYKPRRL FQSVKLHCPK CHLLQEVPHE GDLDIIFQDG ATKTPDVKLQ NTSLYDSKIW TTKNQKGRKV AVHFVKNNGI LPLSNECLLL IEGGTLSEIC KLSNKFNSVI PVRSGHEDLE LLDLSAPFLI QGTIHHYGCK QCSSLRSIQN LNSLVDKTSW IPSSVAEALG IVPLQYVFVM TFTLDDGTGV LEAYLMDSDK FFQIPASEVL MDDDLQKSVD MIMDMFCPPG IKIDAYPWLE CFIKSYNVTN GTDNQICYQI FDTTVAEDVI (336-634 aa encoded by BC002923)

Target Information

POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Its activity is TERT dependent but it does not increase TERT activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.

Experimental cell research

Koranne R,Brown K,Vandenbroek H,Taylor WR

Fri Apr 15 00:00:00 EDT 2022

Telomere Attrition in Induced Pluripotent Stem Cell-Derived Neurons From ALS/FTD-Related C9ORF72 Repeat Expansion Carriers.

Frontiers in cell and developmental biology

Robinson H,Ali SI,Diaz-Hernandez ME,Lopez-Gonzalez R

Sat Jul 16 00:00:00 EDT 2022

Vascular senescence in progeria: role of endothelial dysfunction.

European heart journal open

Xu Q,Mojiri A,Boulahouache L,Morales E,Walther BK,Cooke JP

Fri Jul 01 00:00:00 EDT 2022

The spatiotemporal expression of TERT and telomere repeat binding proteins in the postnatal mouse testes.

Andrologia

Kosebent EG,Ozturk S

Thu Apr 01 00:00:00 EDT 2021

Decreased expression of TERT and telomeric proteins as human ovaries age may cause telomere shortening.

Journal of assisted reproduction and genetics

Uysal F,Kosebent EG,Toru HS,Ozturk S

Mon Feb 01 00:00:00 EST 2021

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients.

Aging

Chen J,Liu Z,Wang H,Qian L,Li Z,Song Q,Zhong G

Wed Feb 10 00:00:00 EST 2021

The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse.

Experimental gerontology

Kosebent EG,Uysal F,Ozturk S

Tue Sep 01 00:00:00 EDT 2020

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation.

Asian Pacific journal of cancer prevention : APJCP

Assani G,Yessoufou A,Xiong Y,Segbo J,Yu X,Zhou F,Zhou Y

Sat Jun 01 00:00:00 EDT 2019

Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer.

Scientific reports

Yang H,Zhang H,Zhong Y,Wang Q,Yang L,Kang H,Gao X,Yu H,Xie C,Zhou F,Zhou Y

Tue Feb 14 00:00:00 EST 2017

Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer.

Scientific reports

Yang H,Zhang H,Zhong Y,Wang Q,Yang L,Kang H,Gao X,Yu H,Xie C,Zhou F,Zhou Y

Tue Feb 14 00:00:00 EST 2017

Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.

Journal of Cancer

Yang H,Wu L,Ke S,Wang W,Yang L,Gao X,Fang H,Yu H,Zhong Y,Xie C,Zhou F,Zhou Y

Tue Jun 21 00:00:00 EDT 2016

Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.

Journal of Cancer

Yang H,Wu L,Ke S,Wang W,Yang L,Gao X,Fang H,Yu H,Zhong Y,Xie C,Zhou F,Zhou Y

Tue Jun 21 00:00:00 EDT 2016

CGGBP1 phosphorylation constitutes a telomere-protection signal.

Cell cycle (Georgetown, Tex.)

Singh U,Maturi V,Jones RE,Paulsson Y,Baird DM,Westermark B

Thu Sep 04 00:00:00 EDT 2014

CGGBP1 phosphorylation constitutes a telomere-protection signal.

Cell cycle (Georgetown, Tex.)

Singh U,Maturi V,Jones RE,Paulsson Y,Baird DM,Westermark B

Thu Sep 04 00:00:00 EDT 2014

Evidence for multiple forms and modifications of human POT1.

DNA repair

Singh U,Maturi V,Westermark B

Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: DKFZp586D211; hPot1; mPot1; POT 1; POT-1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog; Protection of telomeres protein 1

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Gene Aliases: 1500031H18Rik; AI851169; CMM10; GLM9; HPOT1; POT1; Pot1a

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UniProt ID: (Human) Q9NUX5, (Mouse) Q91WC1

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Entrez Gene ID: (Human) 25913, (Mouse) 101185

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Function(s)

protein binding telomerase inhibitor activity DEAD/H-box RNA helicase binding telomeric DNA binding single-stranded telomeric DNA binding G-rich strand telomeric DNA binding DNA binding DNA metabolism protein

Process(es)

telomere maintenance via telomerase telomere capping telomere assembly positive regulation of telomere maintenance via telomerase DNA duplex unwinding positive regulation of helicase activity positive regulation of telomerase activity negative regulation of telomerase activity positive regulation of DNA strand elongation establishment of protein localization to telomere telomere maintenance negative regulation of telomere maintenance via telomerase chromosome organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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POT1 Polyclonal Antibody

POT1 Antibody (10581-1-AP) in IHC (P)

Product Details

10581-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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